背景:小菜蛾(L.)是世界范围内十字花科作物的破坏性害虫,已对许多杀虫剂产生了抗药性。在这里,我们研究了继承的模式,交叉电阻剖面,以及来自日本的P.xylostella田间菌株对甲氨基阿维菌素苯甲酸盐的抗性的潜在机制。
结果:现场收集的木菜鸟种群,与实验室易感菌株相比,在杀虫剂生物测定中发现对甲氨基阿维菌素苯甲酸酯具有很强的抗性(>150倍)。遗传分析表明,抗性是常染色体遗传的,隐性性状,并且由单个或几个紧密相连的基因座赋予。甲氨基阿维菌素苯甲酸酯抗性菌株也表现出对阿维菌素的抗性,lepimectin,chloantraniliprole,卢芬隆,spinetoram,吲哚沙卡威,氟虫腈,狄氏剂,硫丹和氯氟氰菊酯,展示了一个显著的多电阻曲线。使用解毒酶抑制剂的杀虫剂生物测定法显示,胡椒基丁醚(PBO)使甲氨基阿维菌素苯甲酸酯在抗性菌株中的毒性增加了10倍,表明细胞色素P450单加氧酶可能参与阿维菌素抗性。此外,阿维菌素主要受体的克隆和测序,GluCl通道,揭示了抗性菌株中不存在靶位点突变。
结论:我们关于日本小菜鸟菌株对甲氨基阿维菌素苯甲酸酯的遗传方式和抗性机制的数据为制定区域抗性管理策略提供了基础。然而,该菌株对可用于控制的各种其他杀虫剂类别的高水平表型抗性说明了与可持续控制该重要害虫相关的挑战。本文受版权保护。保留所有权利。
BACKGROUND: Plutella xylostella (L.) is a destructive pest of cruciferous crops worldwide that has evolved resistance to many insecticides. Here we examined the mode of inheritance, cross-resistance profile, and potential mechanisms of emamectin benzoate resistance in a field-derived strain of P. xylostella from Japan.
RESULTS: A field-collected population of P. xylostella, was found to exhibit strong (> 150-fold) resistance to emamectin benzoate in insecticide bioassays when compared with a laboratory susceptible strain. Genetic analysis showed that resistance is inherited as an autosomal, recessive trait, and is conferred by a single or a few closely linked loci. The emamectin benzoate resistant strain also exhibited resistance to abamectin, lepimectin, chlorantraniliprole, lufenuron, spinetoram, indoxacarb, fipronil, dieldrin, endosulfan and lambda-cyhalothrin, demonstrating a remarkable multi-resistance profile. Insecticide bioassays employing inhibitors of detoxification enzymes revealed that piperonyl butoxide (PBO) increased the toxicity of emamectin benzoate in the resistant strain by ten-fold indicating the potential involvement of cytochrome P450 monooxygenases in avermectin resistance. Furthermore, cloning and sequencing of the primary receptor of
avermectins, the GluCl channel, revealed the absence of target-site mutations in the resistant strain.
CONCLUSIONS: Our data on the mode of inheritance and mechanisms of resistance to emamectin benzoate in a P. xylostella strain from Japan provide a foundation for the development of regional resistance management strategies. However, the high levels of phenotypic resistance in this strain to a diverse range of other insecticide classes available for control illustrate the challenges associated with the sustainable control of this important pest. © 2023 Society of Chemical Industry.