■Pholiotanameko是一种广泛食用的食用菌。本研究集中在Pholiotanameko的两个关键发育阶段,即,菌丝体和子囊孢子。本研究的目的是研究Pholiotanameko生长过程中微生物多样性和群落结构的变化,并通过代谢分析优势菌株对各自生境的适应性。
■具体来说,我们对从这些阶段获得的样品进行了16SrRNA基因(Illumina)的第二代测序.此外,我们分离并表征了Pholiotanameko中存在的内生菌,重点研究优势内生菌属对自溶的影响。我们还进行了代谢途径分析。
■结果揭示了Pholiotanameko内生真菌的578,414个有效序列。在门一级,优势类群是担子菌,子囊,动物园,和粘菌.在属一级,观察到的主要类群是荷叶草,Inocibe,镰刀菌,和Hortiboletus.对于内生细菌,我们获得了458,475个有效序列。优势门是变形杆菌,TM6,Firmicutes,和拟杆菌,而优势属是Edaphobacter,黄单胞菌,伯克霍尔德利亚,和假单胞菌.此外,我们使用16SrDNA鉴定了Pholiotanameko中的分离菌株,其中大多数被发现属于假单胞菌属,恶臭假单胞菌是最普遍的菌株。研究结果表明,恶臭假单胞菌菌株能够减缓可溶性蛋白质的分解,并部分抑制Pholiotanameko中产生超氧阴离子自由基的代谢过程,从而减少自溶。此外,我们的结果表明,钼酶介导的厌氧氧化磷酸化反应是恶臭假单胞菌菌株的主要能量代谢途径。这表明钼辅因子合成途径可能是Pholiotanameko适应其复杂多样生境的主要机制。
UNASSIGNED: Pholiota nameko is a widely consumed edible fungus. This study focuses on two crucial developmental stages of Pholiota nameko, namely, mycelium and ascospores. The objectives of this research were to investigate changes in microbial diversity and community structure during the growth of Pholiota nameko and to analyze the adaptability of the dominant strains to their respective habitats through metabolic.
UNASSIGNED: Specifically, we conducted second-generation sequencing of the 16S rRNA gene (Illumina) on samples obtained from these stages. In addition, we isolated and characterized endophytes present in Pholiota nameko, focusing on examining the impact of dominant endophyte genera on
autolysis. We also conducted a metabolic pathway analysis.
UNASSIGNED: The results unveiled 578,414 valid sequences of Pholiota nameko endophytic fungi. At the phylum level, the dominant taxa were Basidiomycota, Ascomycota, Zoopagomycota, and Mucoromycota. At the genus level, the dominant taxa observed were Pholiota, Inocybe, Fusarium, and Hortiboletus. For endophytic bacteria, we obtained 458,475 valid sequences. The dominant phyla were Proteobacteria, TM6, Firmicutes, and Bacteroidetes, while the dominant genera were Edaphobacter, Xanthomonas, Burkholderia, and Pseudomonas. Moreover, we identified the isolated strains in Pholiota nameko using 16S rDNA, and most of them were found to belong to the genus Pseudomonas, with Pseudomonas putida being the most prevalent strain. The findings revealed that the Pseudomonas putida strain has the ability to slow down the breakdown of soluble proteins and partially suppress the metabolic processes that generate superoxide anion radicals in Pholiota nameko, thereby reducing
autolysis. Additionally, our results demonstrated that molybdenum enzyme-mediated anaerobic oxidative phosphorylation reactions were the primary energy metabolism pathway in the Pseudomonas putida strain. This suggests that the molybdenum cofactor synthesis pathway might be the main mechanism through which Pholiota nameko adapts to its complex and diverse habitats.