目的:本研究旨在研究在诱导的1-骨壁缺损模型中,内部渗透(IMP)和1%褪黑素(MLN)凝胶对自体骨移植(ABG)重塑过程的影响。
方法:在8只比格犬的前磨牙P1,P2,P3和P4(每侧)的远端产生了64个由骨内引起的下颌骨缺损。在每个缺损中都开始结扎引起的牙周炎。然后将缺陷分为4组。I组仅采用开放皮瓣清创术(OFD)治疗,II组采用OFD/ABG治疗,III组采用OFD/IMP/ABG治疗,IV组用OFD/ABG/IMP/1%MLN凝胶治疗。研究参数是骨填充,组织学分析,2周(2W)和8周(8W)时间间隔的内皮型一氧化氮合酶(eNOS)表达和免疫组织化学评估。
结果:在8W时,在骨填充量和eNOS表达方面,所有组之间均存在显着差异(P<.001)。骨填充百分比为55.5%,22.3%,16.8%,在IV组中为0%,III,II,而我,分别。IV组eNOS表达分别为1.68±0.06、8.43±0.04、16.80±0.17和1.97±0.07,III,II,而我,分别。结果与第IV组一致。
结论:根据这些初步结果,用IMP和1%MLN凝胶增强的ABG治疗的缺陷显示出更大量的骨填充和eNOS表达降低。因此,高度建议这种组合作为ABG的辅助。
OBJECTIVE: This study was designed to investigate the effect of intramarrow penetration (IMP) and 1% melatonin (MLN) gel on the remodelling process of autogenous bone graft (ABG) in an induced 1-osseous wall defect model.
METHODS: Sixty-four intrabony induced mandibular defects were created on the distal side of premolars-P1, P2, P3, and P4 (on each side)-in 8 beagle dogs. A ligature-induced periodontitis was initiated in each defect. Defects were then divided into 4 equal groups. Group I was treated with open-flap debridement (OFD) alone, group II was treated with OFD/ABG, group III was treated with OFD/IMP/ABG, and group IV was treated with OFD/ABG/IMP/1% MLN gel. The study parameters were bone fill, histologic analysis, and immunohistochemical evaluation of endothelial nitric oxide synthase (eNOS) expression at 2-week (2W) and 8-week (8W) time intervals.
RESULTS: At 8W, significant differences were revealed amongst all groups regarding the amount of bone fill and eNOS expressions (P < .001). Bone fill percentages were 55.5%, 22.3%, 16.8%, and 0% in groups IV, III, II, and I, respectively. eNOS expressions were 1.68 ± 0.06, 8.43 ± 0.04, 16.80 ± 0.17, and 1.97 ± 0.07 in groups IV, III, II, and I, respectively. The favourable results were in line with group IV.
CONCLUSIONS: According to these preliminary results, defects treated by ABG augmented with IMP and 1% MLN gel revealed a greater amount of bone fill and reduced eNOS expression. This combination is therefore highly suggested as an adjunct to ABG.