极光激酶B(AURKB),在有丝分裂过程中必不可少的调节剂,已通过各种研究表明,在癌症的发展和进展中具有重要作用。然而,具体机制仍然知之甚少。这项研究,因此,旨在阐明AURKB在多种癌症类型中的多方面作用。这项研究利用生物信息学技术来检查转录本,蛋白质,启动子甲基化和AURKB的突变水平。该研究进一步分析了AURKB与预后等因素之间的关联,病理阶段,生物学功能,免疫浸润,肿瘤突变负荷(TMB)和微卫星不稳定性(MSI)。此外,收集50例肾透明细胞癌及其癌旁正常组织的免疫组化染色数据,验证两种组织中AURKB蛋白表达的差异。结果表明,AURKB在大多数癌症中高表达,AURKB的蛋白质水平及其启动子的甲基化水平因癌症类型而异。生存分析显示,AURKB与12种癌症类型的总生存率和11种癌症类型的无进展生存率相关。在10种不同癌症的晚期阶段检测到AURKB水平升高。AURKB通过其对细胞周期调节以及炎症和免疫相关途径的影响对癌症进展具有潜在影响。我们观察到AURKB和免疫细胞浸润之间有很强的关联,免疫调节因子,TMB和MSI。重要的是,我们证实AURKB蛋白在肾透明细胞癌(KIRC)中高表达。我们的研究表明,AURKB可能是泛癌症和KIRC的潜在生物标志物。
Aurora kinase B (AURKB), an essential regulator in the process of mitosis, has been revealed through various studies to have a significant role in cancer development and progression. However, the specific mechanisms remain poorly understood. This study, therefore, seeks to elucidate the multifaceted role of AURKB in diverse cancer types. This study utilized bioinformatics techniques to examine the transcript, protein, promoter methylation and mutation levels of AURKB. The study further analysed associations between AURKB and factors such as prognosis, pathological stage, biological function, immune infiltration, tumour mutational burden (TMB) and microsatellite instability (MSI). In addition, immunohistochemical staining data of 50 cases of renal clear cell carcinoma and its adjacent normal tissues were collected to verify the difference in protein expression of AURKB in the two tissues. The results show that AURKB is highly expressed in most cancers, and the protein level of AURKB and the methylation level of its promoter vary among cancer types. Survival analysis showed that AURKB was associated with overall survival in 12 cancer types and progression-free survival in 11 cancer types. Elevated levels of AURKB were detected in the advanced stages of 10 different cancers. AURKB has a potential impact on cancer progression through its effects on cell cycle regulation as well as inflammatory and immune-related pathways. We observed a strong association between AURKB and immune cell infiltration, immunomodulatory factors, TMB and MSI. Importantly, we confirmed that the AURKB protein is highly expressed in kidney renal clear cell carcinoma (KIRC). Our study reveals that AURKB may be a potential biomarker for pan-cancer and KIRC.