■芦笋地上部分和根部分提取物的纳米乳液制剂的制备和表征。
■芦笋属以其抗菌和抗癌活性而闻名,然而,本研究制备的水醇提取物冻干粉,表现出有限的水溶性,限制其治疗应用。因此,将其植物化学物质封装到纳米乳液中被提议作为提高水溶性的解决方案,并促进其临床翻译。
■通过HPLC和LC-MS分析鉴定了芦笋的地上部分和根部分的提取物组成。通过均质化制备纳米乳液,其中蓖麻油:磷酸盐缓冲盐水(10mM,pH7.4):Tween80:PEG600,比例分别为10:5:2.5:2.5。对纳米乳液配方的粒径进行了表征,多分散指数(PDI),zeta电位,TEM,粘度和pH。然后,评估了纳米乳液制剂与纯植物制剂的抗菌和抗癌活性。
■提取物的分析确定了几种类黄酮,酚类物质,以及据报道具有抗菌和抗癌活性的皂苷。纳米乳液制剂单分散,液滴尺寸为80.27±2.05至111.16±1.97nm,和多分散指数≤0.3。与它们的纯植物提取物对应物相比,纳米乳液制剂显著增强了抗菌(引起皮肤和牙齿软组织感染的多药抗性细菌)和抗癌(HuH7、HEPG2、H460和HCT116)活性。
■使用纳米递送系统作为植物化学物质的载体可能是增强其药理活性的有效策略,克服他们的局限性,并最终增加其临床应用的潜力。
UNASSIGNED: Preparation and characterization of nano-emulsion formulations for Asparagus densiflorus aerial and root parts extracts.
UNASSIGNED: Genus Asparagus is known for its antimicrobial and anticancer activities, however, freeze dried powder of aqueous - alcoholic extract prepared in this study, exhibited a limited water solubility, limiting its therapeutic application. Thus, encapsulation of its phytochemicals into nano-emulsion is proposed as a solution to improve water solubility, and facilitate its clinical translation.
UNASSIGNED: the composition of extracts for both aerial and root parts of Asparagus densiflorus was identified by HPLC and LC-MS analysis. Nano-emulsion was prepared via homogenization where a mixture of Castor oil: phosphate buffered saline (10 mM, pH 7.4): Tween 80: PEG 600 in a ratio of 10: 5: 2.5: 2.5, respectively. Nano-emulsion formulations were characterized for particle size, polydispersity index (PDI), zeta potential, TEM, viscosity and pH. Then, the antibacterial and anticancer activities of nano-emulsion formulations versus their pure plant counterparts was assessed.
UNASSIGNED: The analysis of extracts identified several flavonoids, phenolics, and saponins which were reported to have antimicrobial and anticancer activities. Nano-emulsion formulations were monodispersed with droplet sizes ranging from 80.27 ± 2.05 to 111.16 ± 1.97 nm, and polydispersity index ≤0.3. Nano-emulsion formulations enhanced significantly the antibacterial (multidrug resistant bacteria causing skin and dental soft tissues infections) and anticancer (HuH7, HEPG2, H460 and HCT116) activities compared to their pure plant extract counterparts.
UNASSIGNED: Employing a nano-delivery system as a carrier for phytochemicals might be an effective strategy to enhance their pharmacological activity, overcome their limitations, and ultimately increase their potential for clinical applications.