目的:本研究评估了在植入新型层状聚乙醇酸(PGA)材料以替代大鼠气管中的临界尺寸缺损后,气管内施用碱性成纤维细胞生长因子(bFGF)对气管愈合的影响。
方法:用PGA覆盖大鼠颈气管的临界尺寸缺损。将蒸馏水(DW)或3.125、6.25、12.5或25µgbFGF施用于气管2周(五组每组n=6)。纤毛再生区,纤毛搏动频率,测量PGA中心的纤毛运输功能。为了检查气管内施用bFGF的潜在副作用,对右下叶进行病理评估。
结果:研究期间所有大鼠均存活。组织学检查显示2周后PGA材料上的纤毛上皮化。支气管镜检查显示,在施用高浓度bFGF(12.5和25µg)后,由于肉芽形成引起的狭窄。与DW组相比,给予3.125、6.25、12.5和25µgbFGF的组再生纤毛面积明显更大(15.2%,27.0%,41.3%,33.1%,和31.0%,分别为;p=0.00143),改善纤毛搏动频率(7.10、8.18、10.10、9.50和9.50Hz,分别),和改善纤毛运输功能(6.40、9.54、16.89、16.41和14.29µm/sec,分别)。右下叶的病理检查显示肺纤维化和增生,并伴有高浓度的bFGF(12.5和25µg)。
结论:在植入人工气管后,以6.25µg的最佳剂量气管内施用bFGF可有效促进气管再生。
OBJECTIVE: This study evaluated the effect of intratracheal administration of basic fibroblast growth factor (bFGF) on tracheal healing following implantation of a novel layered polyglycolic acid (PGA) material to replace a critical-size defect in rat trachea.
METHODS: A critical-size defect in the rat cervical trachea was covered with PGA. Distilled water (DW) or 3.125, 6.25, 12.5 or 25 µg bFGF was administered into the trachea for 2 weeks (n = 6 for each of 5 groups). Regenerated areas of cilia, ciliary beat frequency and ciliary transport function (CTF) in the centre of the PGA were measured. To examine potential side effects of intratracheal administration of bFGF, the right lower lobe was pathologically evaluated.
RESULTS: All rats survived during the study period. Histological examination showed ciliated epithelization on the PGA material after 2 weeks. Bronchoscopy revealed stenosis due to granulation following administration of high concentrations of bFGF (12.5 and 25 µg). Compared with the DW group, groups administered 3.125, 6.25, 12.5 and 25 µg bFGF had significantly larger areas of regenerated cilia (15.2%, 27.0%, 41.3%, 33.1% and 31.0%, respectively; P = 0.00143), improved ciliary beat frequency (7.10, 8.18, 10.10, 9.50 and 9.50 Hz, respectively), and improved CTS (6.40, 9.54, 16.89, 16.41 and 14.29 µm/sec, respectively). Pathological examination of the right lower lobe revealed pulmonary fibrosis and hyperplasia with high concentrations of bFGF (12.5 and 25 µg).
CONCLUSIONS: Intratracheal administration of bFGF effectively promoted tracheal regeneration at an optimal dose of 6.25 µg following implantation of an artificial trachea.