药用植物因其对各种微生物的抗微生物特性而被广泛使用。Arisaemadracontium是一种熟悉的药用植物,分析并使用其芽的不同部分(包括叶和茎)的提取物合成银纳米颗粒(AgNPs)。Further,不同溶剂提取物如乙酸乙酯的抗菌活性,正己烷,乙醇,甲醇,和氯仿提取物进行了分析。使用硝酸银水溶液制备AgNP,并评估其对多药耐药(MDR)和非多药耐药细菌的抗菌活性。通过扫描电子显微镜(SEM)对AgNPs进行表征,透射电子显微镜(TEM),紫外可见光谱,傅里叶变换红外(FTI),和X射线衍射方法。叶子提取物含有单宁,黄酮类化合物,萜类化合物,和类固醇,而生物碱,皂甙,和糖苷未被检测到。茎提取物含有生物碱,单宁,黄酮类化合物,皂甙,类固醇,和糖苷,而萜类未观察到。在本研究中从茎和叶提取物合成的AgNPs具有球形形状,并且如在TEM中可见的,尺寸范围分别为1-50nm和20-500nm。叶提取物制备的AgNP显示出明显更高的活性,即与茎衍生的纳米颗粒相比,针对MDR菌株的27.75mm±0.86,即24.33±0.33,通过比较可以归因于其植物化学成分差异的抑制区。急性毒性试验证实,当剂量为100mg/kg时,没有观察到死亡,这证实了当以低量使用时AgNP是无毒的。结论是,与化学物质相比,a的叶提取物可用于对抗致病菌,具有经济和健康效益。
Medicinal plants have been widely used for their antimicrobial properties against various microorganisms. Arisaema dracontium a familiar medicinal plant, was analyzed and silver nanoparticles (AgNPs) were synthesized using extracts of different parts of its shoot including leaves and stem. Further, the antimicrobial activity of different solvent extracts such as ethyl acetate, n-hexane, ethanol, methanol, and chloroform extracts were analyzed. AgNPs were prepared using aqueous silver nitrate solution and assessed their antibacterial activity against multidrug-resistant (MDR) and Non-multidrug-resistant bacteria. The characterization of AgNPs was done by Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), UV-visible spectroscopy, Fourier Transform Infrared (FTI), and X-ray Diffraction approaches. The leaf extract contained Tannins, Flavonoids, Terpenoids, and Steroids while Alkaloids, Saponins, and Glycosides were undetected. The stem extract contained Alkaloids, Tannins, Flavonoids, Saponins, Steroids, and Glycosides while Terpenoids were not observed. The AgNPs synthesized from stem and leaf extracts in the current study had spherical shapes and ranged in size from 1 to 50 nm and 20-500 nm respectively as were visible in TEM. The leaf extract-prepared AgNPs showed significantly higher activities i.e., 27.75 mm ± 0.86 against the MDR strains as compared to the stem-derived nanoparticles i.e., 24.33 ± 0.33 by comparing the zones of inhibitions which can be attributed to the differences in their phytochemical constituents. The acute toxicity assay confirmed that no mortality was noticed when the dosage was 100 mg per kg which confirms that the confirms that the AgNPs are not toxic when used in low quantities. It is concluded that leaf extract from A. dracontium could be used against pathogenic bacteria offering economic and health benefits compared to the chemical substances.