■关于肝细胞癌(HCC)中载脂蛋白A1(APOA1)的当前知识是零散的,甚至是矛盾的。需要进行多维度分析,以全面阐明其价值和潜在机制。
■我们收集了49个RNA-seq数据集,公开的40种细胞系类型数据和70种scRNA泛癌症数据集,包括17个HCC数据集(1754个肿瘤样本),并从我们的诊所独立登记了73对HCC组织和516份血液样本。使用密集数据挖掘分析了APOA1对HCC肿瘤微环境(TME)的影响。甲基化测序,流式细胞术,定量PCR,westernblot,进行了免疫组织化学和临床化学测定,以进行湿实验研究。
■APOA1本体论指纹表明它在HCC中起着各种重要的生物学作用,主要参与胆固醇流出。组织学上的一致发现,血清学,临床随访显示,高APOA1是HCC的良好预后指标。在临床样品中发现APOA1启动子区域的超甲基化,这与HCC中APOA1的降低一致。细胞周期,DNA复制,错配修复途径,在HCCAPOA1高亚组中观察到的肿瘤细胞增殖较少。APOA1的良好免疫调节能力显示出有趣的发现:APOA1与抗肿瘤免疫细胞(NK,CD8+T细胞)和与发挥免疫抑制作用的免疫细胞呈负相关,包括M2巨噬细胞。
■这是利用生物信息学和实验对APOA1进行的综合多维探索。基于APOA1全景探索的HCCTME的预后价值和抗肿瘤作用都为将来的HCC评估和干预提供了新的潜在临床目标。
UNASSIGNED: Current knowledge on apolipoprotein A1 (
APOA1) in hepatocellular carcinoma (HCC) is fragmented and even contradictory. Multi-dimensional analyses are required to comprehensively elucidate its value and underlying mechanism.
UNASSIGNED: We collected 49 RNA-seq datasets, 40 cell line types data and 70 scRNA pan-cancer datasets public available, including 17 HCC datasets (1754 tumor samples), and enrolled 73 pairs of HCC tissue and 516 blood samples independently from our clinics.
APOA1 impacting on the HCC tumor microenvironment (TME) was analyzed using intensive data mining. Methylation sequencing, flow cytometry, quantitative PCR, western blot, immunohistochemistry and clinical chemistry assays were conducted for wet experimental investigation.
UNASSIGNED: The APOA1 ontology fingerprint indicated that it played various crucial biological roles in HCC, primarily involved in cholesterol efflux. Consistent findings at histology, serology, and clinical follow-up revealed that high
APOA1 was a good prognosis indicator of HCC. Hypermethylation in the APOA1 promoter region was found in clinical samples which is in accordance with the reduction of
APOA1 in HCC. The cell cycle, DNA replication, mismatch repair pathways, and tumor cell proliferation were less observed in the HCC APOA1high subgroup. The favorable immunoregulatory abilities of
APOA1 showed interesting findings: a positive correlation between APOA1 and anti-tumor immune cells (NK, CD8+ T cells) and a negative association with immune cells exerting immunosuppressive effects, including M2 macrophages.
UNASSIGNED: This is an integrative multidimensional exploration of APOA1 using bioinformatics and experiments. Both the prognostic value and anti-tumor effects based on APOA1 panoramic exploration in the HCC TME demonstrate a new potential clinical target for HCC assessment and intervention in the future.