在印度东部,葛根的块茎(威尔德。)DC。因其广泛的药用和营养价值而被种族社区使用,特别是恢复牲畜健康和治疗蠕虫病。该研究旨在评估结核分枝杆菌作为驱虫药的民族兽医学重要性,验证其无毒性质,并通过计算机分子对接技术鉴定最有效的植物成分。使用八个定量指标对从185个线人收集的民族医学数据进行了定量分析,以突出使用多样性和植物中最常用的部分。采用的某些指数得分很高,如使用值(UV=0.52),保真度水平(FL=68.42%)和组织重要性值(TIV=1)清楚地说明了有关块茎部分用于治疗兽用动物肠道蠕虫病的医学营养益处的种族医学线索。基于这个民族指导的线索,块根已经通过对总酚的估计进行了化学分析研究,黄酮类化合物,单宁和生物碱,以及HPLC和GC-MS分析。通过对畜禽鸟类的某些蠕虫的体外研究,用块茎提取物评估了驱虫性能,它显示了对测试的寄生虫,即棉草,四角拉利蒂娜和塞洛里亚。使用Vero细胞和BALB/c小鼠通过体外和体内方法进行块茎提取物的毒性评估。即使在较高的实验剂量下,也观察到所研究的块茎提取物的无毒性质。在通过GC-MS分析鉴定的12种植物化合物中,一种化合物[Morphinan-4,5-环氧-3,6-二醇,6-(7-硝基苯并呋喃-4-基)氨基-]在最低结合能值的成本中表现出最佳的结合构象,其中包括一种抗炎药,一种抗氧化剂,和四种驱虫蛋白.我们的研究结果非常令人鼓舞,可以进一步深入评估这种块茎药物,以开发驱虫兽药。
In eastern India, the tubers of Pueraria tuberosa (Willd.) DC. are used by the ethnic communities for its wide range of medicinal and nutritional value, especially to rejuvenate livestock health and to treat helminthiasis. The study is aimed to evaluate the ethnoveterinary medicinal importance of P. tuberosa as anthelmintic, to verify its nontoxic nature and identify the most potent phytoconstituents aided by in silico molecular docking technique. Ethnomedicinal data collected from 185 informants were quantitatively analyzed employing eight quantitative indices to highlight the use diversity and most frequently used part of the plant. High scores of certain indices employed, such as Use Value (UV = 0.52), Fidelity Level (FL = 68.42%) and Tissue Importance Value (TIV = 1) clearly illustrate an ethnomedicinal lead regarding medico-nutritional benefits of the tuber part used against intestinal helminthic diseases of veterinary animals. Based on this ethno-guided lead, root tuber has been investigated for its chemical profiling by the estimation of total phenolics, flavonoids, tannins and alkaloids, along with HPLC and GC-MS analyses. Anthelmintic property was evaluated with the tuber extracts by in vitro studies on some helminths of livestock and poultry birds, and it showed promising results against the tested parasites namely Cotylophoron cotylophorum, Raillietina tetragona and Setaria cervi. Toxicity assessments of tuber extract through in vitro and in vivo methods were performed using Vero cells and BALB/c mice. Nontoxic nature of the studied tuber extract was observed even in higher experimental doses. Out of 12 phytocompounds identified by GC-MS analysis, one compound [Morphinan-4,5-epoxy-3,6-di-ol,6- (7-nitrobenzofurazan-4-yl) amino-] exhibited the best binding conformations in cost of the lowest binding energy values with six target proteins that include one anti-inflammatory, one antioxidant, and four anthelmintic proteins. The findings of our study are found very encouraging to evaluate this tuber drug furthermore intensively towards the development of anthelmintic veterinary medicine.