目的:本研究旨在确定生姜提取物(GE)的化学成分,评估GE对生姜青枯病病原菌青枯病菌的抗菌活性,并筛选其作用机制。
结果:使用超高效液相色谱和串联质谱法共鉴定了393个化合物。抗菌试验表明,GE对青枯菌具有较强的抗菌活性,杀菌效果呈剂量依赖性。青枯菌的最低抑菌浓度和最低杀菌浓度分别为3.91和125mg/ml,分别。葛根破坏了青枯菌的细胞膜通透性和完整性,导致细胞内容泄漏,如电解质,核酸,蛋白质,胞外三磷酸腺苷和外多糖。此外,随着GE浓度的增加,青枯菌细胞琥珀酸脱氢酶和碱性磷酸酶的活性逐渐降低。扫描电子显微镜分析显示,GE处理改变了茄尾R.细胞的形态。进一步的实验表明,GE延缓或减缓了生姜上青枯病的发生。
结论:GE对青枯菌有明显的抗菌作用,抗菌效果是浓度依赖性的。GE治疗改变了形态,破坏膜的渗透性和完整性,降低青枯菌关键酶活性并抑制毒力因子EPS的合成。GE显著控制了生姜侵染过程中的青枯病。
结论:这项研究深入了解了GE对青枯菌的抗菌机制,这将为GE开辟一个新的应用领域。
OBJECTIVE: The current study aimed to determine the chemical compositions of ginger extract (GE) and to assess the antibacterial activities of GE against the ginger bacterial wilt pathogen Ralstonia solanacearum and to screen their mechanisms of action.
RESULTS: A total of 393 compounds were identified by using ultra-performance liquid chromatography and tandem-mass spectrometry. The antibacterial test indicated that GE had strong antibacterial activity against R. solanacearum and that the bactericidal effect exhibited a dose-dependent manner. The minimum inhibitory concentration and minimum bactericidal concentration of R. solanacearum were 3.91 and 125 mg/ml, respectively. The cell membrane permeability and integrity of R. solanacearum were destroyed by GE, resulting in cell content leakage, such as electrolytes, nucleic acids, proteins, extracellular adenosine triphosphate and exopoly saccharides. In addition, the activity of cellular succinate dehydrogenase and alkaline phosphatase of R. solanacearum decreased gradually with an increase in the GE concentration. Scanning electron microscopy analysis revealed that GE treatment changed the morphology of the R. solanacearum cells. Further experiments demonstrated that GE delayed or slowed the occurrence of bacterial wilt on ginger.
CONCLUSIONS: GE has a significant antibacterial effect on R. solanacearum, and the antibacterial effect is concentration dependent. The GE treatments changed the morphology, destroyed membrane permeability and integrity, reduced key enzyme activity and inhibit the synthesis of the virulence factor EPS of R. solanacearum. GE significantly controlled the bacterial wilt of ginger during infection.
CONCLUSIONS: This research provides insight into the antimicrobial mechanism of GE against R. solanacearum, which will open a new application field for GE.