背景:雪莲(Anacardiaceae)富含精油,以单萜和倍半萜为主,它在传统医学中广泛用于治疗炎症。
目的:本研究的目的是调查在巴西六个州收集的三黄曲霉精油(EOST)的化学成分,评估其在小鼠中的抗炎作用,并分析了叶和茎的组织化学和微观形态。
方法:收集了巴西六个州的terebinthifolia的地上部分,采用加氢蒸馏法提取精油,采用气相色谱-质谱(GC-MS)分析。使用标准试剂进行叶和茎的组织化学和微观形态,光和场发射扫描电子显微镜,超越能量色散X射线光谱。使用角叉菜胶诱导的爪水肿方法评估EOST的抗炎活性和痛觉过敏。
结果:EOST在其产量(0.40%-0.86%)和化学成分:烃单萜(28.76%-47.73%)的六个状态中显示出变化,倍半萜,(31.43%-41.76%),含氧单萜(14.31%-19.57%),和含氧倍半萜(4.87%-14.38%)。α-pine烯和柠檬烯都是五个地区的主要必需成分,除了其中α-phellandrene和柠檬烯是主要成分的一种状态。对叶和茎的微观形态和组织化学进行了全面描述。在体内测试中,所有EOST样本均发挥抗水肿和抗痛觉过敏作用,当在角叉菜胶诱导的爪炎症(机械和热痛觉过敏)模型中测试时,口服剂量为30mg/kg。
结论:我们的结果表明,在巴西六个州收集的EOST样品的化学成分不同,但抗炎和抗痛觉过敏作用不同。这与其组分的协同作用相关,由于具有抗炎作用,因此合作使用该植物。此外,本研究中提出的叶和茎的微观形态和组织化学提供了解剖和微观化学信息,这有助于物种识别。
BACKGROUND: Schinus terebinthifolia Raddi (Anacardiaceae) is rich in essential oil, distinguished by a predominance of monoterpenes and sesquiterpenes, it being widely used in traditional medicine for the treatment of inflammations.
OBJECTIVE: This study\'s objective was to investigate the chemical composition of the essential oil of S. terebinthifolia (EOST) collected in six states of Brazil, evaluate its anti-inflammatory effects in mice, and analyze the histochemistry and micromorphology of leaves and stems.
METHODS: Aerial parts of S. terebinthifolia were collected in six states of Brazil, and the essential oil was extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS). The histochemistry and micromorphology of leaves and stems were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy. The EOST were evaluated for anti-inflammatory activity and hyperalgesia using the carrageenan-induced paw edema methodology.
RESULTS: The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%). Both α-pinene and limonene were predominant constituents of essential in five regions, except for one state where α-phellandrene and limonene were the dominant components. A comprehensive description of the leaf and stem micromorphology and histochemistry was performed. In the in vivo testing, all EOST samples exerted antiedematogenic and anti-hyperalgesic effects, when tested in a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg.
CONCLUSIONS: Our results indicate that the EOST samples collected in six Brazilian states differed in their chemical composition but not their anti-inflammatory and antihyperalgesic effects, which was correlated with the synergistic effect of its components, collaborating the etnhopharmacologycal use of this plant due to its an anti-inflammatory effect. Also, micromorphology and histochemistry of leaves and stems presented in this study provide anatomical and microchemical information, which aids species identification.