目的:研究白藜芦醇(RSV)对卵巢形态学的影响,血浆抗苗勒管激素(AMH)和胰岛素样生长因子1(IGF-1)水平,多囊卵巢综合征(PCOS)大鼠的氧化应激指标。
方法:将46只大鼠随机分为正常对照组(n=12),PCOS模型控制(n=12),罗格列酮(RSG,n=11),和RSV组(n=11)。后三组通过排斥表氢雄酮建立PCOS模型。正常对照组和PCOS模型对照组大鼠灌胃生理盐水,RSG和RSV组大鼠灌胃10mg/(kg·d),RSV3.0mg/(kg·d),分别。治疗4周后,在光学显微镜下观察卵巢组织学,ELISA法测定血浆AMH和IGF-1水平,和谷胱甘肽过氧化物酶(GSH-Px)的活性,使用Ellman检测卵巢组织中的超氧化物歧化酶(SOD)和过氧化氢酶(CAT),Sun和AEBI方法,分别。
结果:治疗4周后,正常对照组与PCOS模型对照组上述指标比较差异均有统计学意义(P<0.05)。用RSG和RSV处理的大鼠在这些参数方面也显示出与模型对照的显著差异(P<0.05)。
结论:RSV能增强卵巢局部抗氧化能力,降低AMH和IGF-1水平,改善PCOS大鼠卵巢组织形态学,表明其在PCOS治疗中的潜在价值。
OBJECTIVE: To investigate the effects of resveratrol (RSV) on ovarian morphology, plasma anti-Müllerian hormone (AMH) and insulin-like growth factor 1 levels (IGF-1), and oxidative stress parameters in rats with polycystic ovary syndrome (PCOS).
METHODS: Forty-six rats were randomly divided into a normal control (n = 12), a PCOS model control (n = 12), a rosiglitazone (RSG, n = 11), and an RSV group (n = 11). The PCOS model was established in the latter three groups by rejection of epidehydroandrosterone. The rats in the normal control and PCOS model control groups were treated by gavage of normal saline and those in the RSG and RSV groups by intragastric administration of RSG at 10 mg/(kg·d) and RSV at 3.0 mg/(kg·d), respectively. After 4 weeks of treatment, the ovarian histology was observed under the light microscope, the levels of plasma AMH and IGF-1 measured by ELISA, and the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) in the ovarian tissue detected using the Ellman, Sun and AEBI methods, respectively.
RESULTS: After a 4-week treatment, statistically significant differences were observed in the above indicators between the normal control and PCOS model control groups (P<0.05). The rats treated with RSG and RSV also showed significant differences in these parameters from the model controls (P<0.05).
CONCLUSIONS: RSV can enhance the local antioxidant capacity of the ovary, reduce the levels of AMH and IGF-1, and improve the morphology of the ovarian tissue in rats with PCOS, indicating its potential value in the treatment of PCOS.