未经授权:刺参属于苔藓植物的独特谱系,具有阐明陆地植物进化的关键特征;然而,刺耳的功能性遗传工具的发展受到了其相对缓慢的配子体生长的阻碍。
UNASSIGNED:为了确定影响刺参配子体发育并可能促进其生长的外部因素,我们评估了几种培养基成分对Anthocerosagrestis的无菌配子体生长的贡献,一种型号的角草.开发了一种利用半自动图像分析的流线型生长测定法,以快速量化和比较在固化培养基上培养四周的组织发育。
未经批准:添加蔗糖,硝酸铵,活性炭,pH值,缓冲和生长调节剂(2,4-二氯苯氧基乙酸,6-苄基氨基嘌呤,和噻二唑)影响配子体组织存活,增长模式,和塔里生长的速度。随后,制定了优化的培养基组成和生长方案,以加速A.agretis配子体生长,与其他先前使用的刺耳菌生长培养基相比,在培养四周时,组织湿重增加了2.1至8.5倍。
UNASSIGNED:我们用于产生有力的起始材料和加速组织再生的方案与推进角参的基因功能表征和基因组编辑有关。
UNASSIGNED: Hornworts belong to a unique lineage of bryophytes with critical traits for elucidating the evolution of land plants; however, the development of functional genetic tools for hornworts has been hampered by their relatively slow gametophytic growth.
UNASSIGNED: To identify the external factors that influence the development of hornwort gametophytes and potentially augment their growth, we evaluated the contributions of several culture medium components on the axenic gametophytic growth of Anthoceros agrestis, a model hornwort. A streamlined growth assay utilizing semiautomated image analysis was developed to rapidly quantify and compare tissue development spanning four weeks of culture on solidified medium.
UNASSIGNED: The addition of sucrose, ammonium nitrate, activated charcoal, pH buffering, and growth regulators (2,4-dichlorophenoxyacetic acid, 6-benzylaminopurine, and thidiazuron) affected gametophyte tissue survival, growth patterns, and the rate of thalli growth. Subsequently, an optimized medium composition and growth regimen for accelerating A. agrestis gametophytic growth were formulated, which at four weeks of culture increased the tissue wet weight by 2.1- to 8.5-fold compared with other previously utilized hornwort growth media.
UNASSIGNED: Our protocol for generating vigorous starting material and accelerated tissue regeneration is pertinent for advancing gene function characterization and genome editing in hornworts.