在本研究中,我们试图阐明K1T的分类位置，喜石芽孢杆菌NCIMB13971T，黄热病杆菌亚种。云南CCTCCAB2010187T，和使用全基因组系统发育分析的TengchogensisDSM23211T。A.kestanbolensisNCIMB13971T的基因组序列在任何数据库中都没有，所以在这项研究中对它进行了测序。从A.kestanbolensisNCIMB13971T基因组获得的16SrRNA基因序列与A.karvacharensisK1T具有99.93％的相似性。平均核苷酸同一性(ANI)，平均氨基酸同一性(AAI)，和A.karvacharensisK1T和A.kestanbolensisNCIMB13971T之间的数字DNA-DNA杂交（DDH）值以及A.flavithermussubsp。云南CCTCCAB2010187T和A.tengchungensisDSM23211T大于物种划分的阈值。目前的结果表明，A.karvacharensisK1T是A.kestanbolensisNCIMB13971T的后来异型同义词；黄热菌亚种。云南CCTCCAB2010187T是TengchogensisDSM23211T的后来异型同义词。
In the present study, we attempted to clarify the taxonomic positions of Anoxybacillus karvacharensis K1T, Anoxybacillus kestanbolensis NCIMB 13971T, Anoxybacillus flavithermus subsp. yunnanensis CCTCC AB2010187T, and Anoxybacillus tengchongensis DSM 23211T using whole-genome phylogenetic analysis. The genome sequence of A. kestanbolensis NCIMB13971T was not available in any database, so it was sequenced in this study. The 16S rRNA gene sequence obtained from the genome of A. kestanbolensis NCIMB13971T had 99.93% similarity with A. karvacharensis K1T. The average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridization (DDH) values between A. karvacharensis K1T and A. kestanbolensis NCIMB13971T and between A. flavithermus subsp. yunnanensis CCTCCAB 2010187T and A. tengchongensis DSM 23211T were greater than the threshold values for species demarcation. The present results indicate that A. karvacharensis K1T is a later heterotypic synonym of A. kestanbolensis NCIMB13971T; A. flavithermus subsp. yunnanensis CCTCCAB 2010187T is a later heterotypic synonym of A. tengchongensis DSM 23211T.
