anisakid线虫的L3幼虫是渔业行业的重要问题,并且通过充当引起过敏的传染因子以及病原体和微生物的潜在载体,对人类健康构成潜在风险。尽管细菌与线虫之间有着密切的关系,但对anisakids微生物群知之甚少。当L3幼虫通过肌肉迁移时,新鲜的鱼可能会被角质层或anisakids肠道中的细菌所污染。因此,细菌接种物将被传播,对鱼的质量有潜在的影响,和可能的临床效果不能被丢弃。研究了从FAO27捕鱼区捕获的鱼类中获得的总共113个L3个体的2,689,113个16SrRNA基因序列。通过1803个代表性操作分类单位(OTU)序列对细菌进行分类学表征。十四个门,31班,52个订单,明确确定了129个家庭和187个属。作为微生物组的一部分,我们发现每个L3个体平均有123个OTU。多样性指数(Shannon和Simpson)表明在OTU水平上细菌的非凡多样性。有一簇由相关细菌定义的anisakids个体(样本),然而,与鱼类宿主或anisakid类群没有显着关系。这表明anisakids中细菌之间的关联或关系,不受鱼类或线虫的影响而存在。与anisakids类群的宿主缺乏关系必须通过细菌OTU或从OTU到门水平的其他分类学水平之间的关联来表达。anisakid线虫中微生物群存在显着的生物学结构关联,表现在从门到属水平的细菌集群中,这也可能是鱼类污染或鱼类捕获地理区域的指标。放线菌,Aquificae,Firmicutes,和变形杆菌是门,其丰度值用于定义此类结构。
L3 larvae of anisakid nematodes are an important problem for the fisheries industry and pose a potential risk for human health by acting as infectious agents causing allergies and as potential vectors of pathogens and microrganisms. In spite of the close bacteria-nematode relationship very little is known of the
anisakids microbiota. Fresh fish could be contaminated by bacteria vectored in the cuticle or in the intestine of
anisakids when the L3 larvae migrate through the muscles. As a consequence, the bacterial inoculum will be spread, with potential effects on the quality of the fish, and possible clinical effects cannot be discarded. A total of 2,689,113 16S rRNA gene sequences from a total of 113 L3 individuals obtained from fish captured along the FAO 27 fishing area were studied. Bacteria were taxonomically characterized through 1803 representative operational taxonomic units (OTUs) sequences. Fourteen phyla, 31 classes, 52 orders, 129 families and 187 genera were unambiguously identified. We have found as part of microbiome an average of 123 OTUs per L3 individual. Diversity indices (Shannon and Simpson) indicate an extraordinary diversity of bacteria at an OTU level. There are clusters of
anisakids individuals (samples) defined by the associated bacteria which, however, are not significantly related to fish hosts or anisakid taxa. This suggests that association or relationship among bacteria in
anisakids, exists without the influence of fishes or nematodes. The lack of relationships with hosts of
anisakids taxa has to be expressed by the association among bacterial OTUs or other taxonomical levels which range from OTUs to the phylum level. There are significant biological structural associations of microbiota in anisakid nematodes which manifest in clusters of bacteria ranging from phylum to genus level, which could also be an indicator of fish contamination or the geographic zone of fish capture. Actinobacteria, Aquificae, Firmicutes, and Proteobacteria are the phyla whose abundance value discriminate for defining such structures.