背景:马氏中原虫蝎子,称为“Quanxie”,是一千多年来以“以毒还毒”战略为基础的中药材,根据《中华人民共和国药典》,目前仍被广泛用于治疗各种疾病。
目的:本研究旨在探讨成年期和幼年期马氏藻蝎子作为中药材料在蛋白质水平上蝎子神经毒素的相似性。
方法:第二种,三龄和四龄,和成年的马氏martensii蝎子被收集用于通过多种战略蛋白质组学表征神经毒素的表达,包括未消化的蝎毒,内肽酶消化,和未消化的蝎子telson提取物用于样品分析。
结果:基于已知的107个蝎子神经毒素,这些毒素来自成年马氏藻蝎子的基因组和转录组学分析,多重战略蛋白质组学首次揭示了神经毒素在telson提取物中比分泌的毒液表现出更高的稳定性。在报告的蝎子神经毒素的转录本中,大约53%,56%,通过未消化的蝎毒检测到66%和78%的神经毒素,内肽酶Arg-C-,Lys-C消化的Telson提取物,和未消化的Telson提取策略,分别。迄今为止,在三龄martensiimesobuthusmartensii蝎中检测到的将近79%的蝎子神经毒素代表了蛋白质组学分析中数量最多的蝎子神经毒素。此外,总共84%的蝎子神经毒素在蛋白质水平上被成功鉴定,和类似的神经毒素表达谱在第二个-,三龄和四龄,而成虫马氏藻蝎子首先由多重战略蛋白质组学揭示。
结论:这些发现首次证明了幼年和成年的马氏藻蝎子与中药材的神经毒素表达谱相似,这将作为进一步分析不同有毒动物毒素的范例。
BACKGROUND: The Mesobuthus martensii scorpions, called as \"Quanxie\", are known Chinese medicinal material base on the \"Combat poison with poison\" strategy for more than one thousand years, and still widely used to treat various diseases according to the Pharmacopoeia of the People\'s Republic of China nowadays.
OBJECTIVE: The study aims to investigate the similarity of scorpion neurotoxins at the protein level between the juvenile and adult Mesobuthus martensii scorpions as Chinese medicine materials.
METHODS: The second-, third- and fourth-instar, and adult Mesobuthus martensii scorpions were collected for the characterization of neurotoxin expression through multiple strategic proteomics, including undigested scorpion venom, endopeptidase-digested, and undigested scorpion telson extract for the sample analysis.
RESULTS: Based on the known 107 scorpion neurotoxins from the genomic and transcriptomic analysis of adult Mesobuthus martensii scorpions, the multiple strategic proteomics first revealed that neurotoxins exhibited more stability in telson extract than secreted venom. In the reported transcripts of scorpion neurotoxins, approximately 53%, 56%, 66% and 78% of neurotoxins were detected through undigested scorpion venom, the endopeptidase Arg-C-, Lys-C-digested telson extract, and undigested telson extract strategies, respectively. Nearly 79% of scorpion neurotoxins detected in third-instar Mesobuthus martensii scorpions represent the largest number of scorpion neurotoxins from proteomic analysis to date. Moreover, a total of 84% of scorpion neurotoxins were successfully identified at the protein level, and similar neurotoxin expression profiles in second-, third- and fourth-instar, and adult Mesobuthus martensii scorpions were first revealed by the multiple strategic proteomics.
CONCLUSIONS: These findings for the first time demonstrate the similar neurotoxin expression profiles between the juvenile and adult Mesobuthus martensii scorpions as Chinese medicinal material, which would serve as a paradigm for further toxin analysis from different venomous animals.