背景:管圆线虫病是由大鼠肺虫广州管圆线虫病引起的人畜共患寄生虫病。广东曲霉的中间宿主是腹足类,蜗牛物种,例如Pomaceacanaliculata,在人类血管圆线虫病的传播中起着关键作用。检测蜗牛中的A.cantonensis感染是流行病学监测和控制管圆线虫病的重要组成部分。
方法:在本研究中,通过从三种蜗牛的颊腔中回收幼虫,开发了一种诊断腹足类动物中广东A.cantonensis感染的新方法。蜗牛的整个颊腔都被拔掉了,并将组织压在两个显微镜载玻片之间以观察是否存在。我们的新方法与传统的肺部显微镜致病性检测方法进行了比较,组织均质化,和人工消化。我们人工感染了160个泪珠,160Cipangopaludinachinensis,和160只绿脓杆菌蜗牛和广东曲霉。然后,4种不同的检测方法被用于在暴露后7,14,21和28天诊断每种蜗牛的感染。
结果:我们发现,使用四种方法检测A.cantonensis幼虫,感染的P.canaliculata蜗牛的百分比没有显着差异。侏儒压法的平均检出率为80%,而肺镜检(81.3%),组织均质化(83.8%),和人工消化(85%)方法的检出率稍高。同样,使用神经根按压检测到的受感染的C.chinensis蜗牛的百分比(80%),组织匀浆(82.1%),与人工消化(83.8%)方法无显著差异。最后,感染的铜绿芽孢杆菌蜗牛的百分比,检测到使用的雷达按压(81.3%),组织均质化(81.9%),与人工消化(81.4%)方法无显著差异。这些结果表明,神经根按压法的检出率与传统的肺显微镜相似,组织均质化,或人工消化方法。
结论:这项研究证明了一种新的方法,用于定性筛选作为广州A.cantonensis(和其他管圆线虫)中间宿主的腹足类动物,为控制人类管圆线虫病提供技术支持,和进一步的研究。
BACKGROUND: Angiostrongyliasis is a zoonotic parasitic disease caused by the rat lungworm
Angiostrongylus cantonensis. The intermediate hosts of A. cantonensis are gastropods, and snail species such as Pomacea canaliculata play a key role in the transmission of human angiostrongyliasis. Detecting A. cantonensis infection in snails is an important component of epidemiological surveillance and the control of angiostrongyliasis.
METHODS: In this study, a new method for diagnosing A. cantonensis infection in gastropods was developed by recovering larvae from the buccal cavity of three snail species. The entire buccal cavity of a snail was extracted, and the tissue was pressed between two microscope slides to observe whether A. cantonensis larvae were present. Our new method was compared with traditional pathogenic detection methods of lung microscopy, tissue homogenization, and artificial digestion. We artificially infected 160 P. canaliculata, 160 Cipangopaludina chinensis, and 160 Bellamya aeruginosa snails with A. cantonensis. Then, the four different detection methods were used to diagnose infection in each snail species at 7, 14, 21, and 28 days post exposure.
RESULTS: We found no significant difference in the percentages of infected P. canaliculata snails using the four methods to detect A. cantonensis larvae. The radula pressing method had a mean detection rate of 80%, while the lung microscopy (81.3%), tissue homogenization (83.8%), and artificial digestion (85%) methods had slightly greater detection rates. Similarly, the percentages of infected C. chinensis snails that were detected using the radula pressing (80%), tissue homogenization (82.1%), and artificial digestion (83.8%) methods were not significantly different. Finally, the percentages of infected B. aeruginosa snails that were detected using the radula pressing (81.3%), tissue homogenization (81.9%), and artificial digestion (81.4%) methods were not significantly different. These results showed that the radula pressing method had a similar detection rate to traditional lung microscopy, tissue homogenization, or artificial digestion methods.
CONCLUSIONS: This study demonstrates a new method for the qualitative screening of gastropods that act as intermediate hosts of A. cantonensis (and other
Angiostrongylus species), provides technical support for the control of human angiostrongyliasis, and furthers research on A. cantonensis.