IL-10和干扰素-γ(IFN-γ)是重要的抗炎和促炎细胞因子,分别,可能参与心血管疾病的发病机制。此外,环境因素,比如X光片,可以调节细胞因子的表达。由于血管造影期间使用X射线,因此,血管造影可能改变细胞因子的表达。
目标:因此,该项目旨在评估心血管患者(有血管狭窄和无血管狭窄)与健康对照组的血清IL-10和IFN-γ水平,以及血管造影对血清细胞因子水平的影响.
方法:这项研究对80名参与者进行,每组20例(健康对照组和无血管狭窄的心血管患者,1血管狭窄和1血管以上狭窄)使用ELISA技术评估IL-10和IFN-γ血清水平。还比较了血管造影前后第2、3和4组的IL-10和IFN-γ血清水平,以探讨该技术对IL-10和IFN-γ血清水平的影响。
结果:IL-10,但不是IFN-γ,健康对照组的血清水平高于所有心血管患者。血管造影术后IL-10和IFN-γ血清水平没有改变,吸烟者与非吸烟者和鸦片消耗者与非鸦片消耗者也没有差异。
结论:由于该结果,可以得出结论,IL-10可以被认为是心血管疾病的合理抑制剂,而与血管造影持续时间和X射线无关,然而,IFN-γ对患有心血管疾病的伊朗患者没有影响。
INTERLEYREKIN-10(IL-10)TA干扰素-γ(IFN-γ)即vazhlyvymy,vidpovidno,质子-taprozapal\'nymytsy-tokinamy,iakimozhut\'bratyuchast\'upatogenezisertsevo-sudynnykhzakhoriuvan\'。克里姆·托戈,faktorynavkolysh-n\'ogoseredovyshcha-taki,Iakrentgenivs\'kevyprominiuvannia-zdatnimoduliuvatyekspresiiutsytokiniv.Cherezte,shchorentgenivs\'kevyprominiuvanniavykorystovuietTsiaProtsedura,Mozhlyvo,zminiuieekspresiiutsytokiniv.Meta。OttinytyrivniIL-10taIFN-γsyrovatkykroviatakozhefektyangiografiíshchodovmistutsytokinivusyrovattsikrovi.MaterialytaMetody.Doslidzhenniabuloprovedeno认为80uchasnykiv-po20vypadkivukozhniytogrupi(zdo-rovikontrolitaosobyizsertsevo-sudynymyzakhvoriuvanniamybestenozusudyn,izstenozom1sudynyta-nozombil\'shnizh1sudyny)dliaotsinkyrivnivIL-10taIFN-γusyrovattsikrovizadopomogoiumetoduimunofer-mentnogoanalizu.Dliaz'iasuvanniavplyvuproprosseduryangiografiínariven\'IL-10taIFN-γusyrovattsikrovi,vmistobokhtsytokinivtakozhporivniuvalyugrupakh2,3ta4dotatapisliaangiografichogodoslidzhennia.Rezul\'taty.Riven\'IL-10,例如IFN-γ,usyrovattsikrovizdorovykhosibgrupykontroliubuvbil\'shym,nizhuvsikhpatsiientivizsertsevo-sudynnymyzakhvoriuvanniamy.Riven\'IL-10taIFN-γatakozhnevidrizniavsiaukurtsivtaosib,iakinekuriat\',taksamoiakiuspozhyvachivopiutaosib,iakiopiyénevzhyvaiut\'。Vysnovky.Zavdiakyrezul\'tatammozhnazrobytyvysnovok,shchoIL-10mozhnay.movirnovvazhatyingibitoromser-tsevo-sudynnykhzakhvoriuvan\',nezalezhnovidtryvalostiangiografitarentgenografifida,v玩具iakIFN-γnepro-iavliaieefektivuirans\'kykhpatsiientiv,iakistrazhdaiut\'nasertsevo-sudynnizakhvoriuvannia.
IL-10 and interferon-gamma (IFN-γ) are the important anti and pro-inflammatory cytokines, respectively, which may participate in the cardiovascular disease pathogenesis. Additionally, environmental factors, such as the X-ray, can modulate cytokine expression. Due to the fact that X-ray is used during angiography, hence, angiography may alter expression of the cytokines.
OBJECTIVE: Accordingly, this project was aimed to assess IL-10 and IFN-γ serum levels within cardiovascular patients (with and without vessel stenosis) versus healthy controls and also the effects of angiography on the serum levels of the cytokines.
METHODS: This study was performed on the 80 participants, including twenty cases in each group (healthy controls and cardiovascular patients without vessel stenosis, stenosis of 1 vessel and stenosis of more than 1 vessel) to evaluate IL-10 and IFN-γ serum levels using ELISA technique. The IL-10 and IFN-γ serum levels also compared within group 2, 3 and 4 before and after angiography to explore the effects of the technique on the IL-10 and IFN-γ serum levels.
RESULTS: IL-10, but not IFN-γ, serum levels were higher in the healthy controls than all cardiovascular patients. IL-10 and IFN-γ serum levels were not altered after angiography and also were not differ in the smoker versus non- smoker and opium consuming versus non-opium consuming participants.
CONCLUSIONS: Due to the results it may be concluded that IL-10 can be considered as a plausible inhibitor of cardio- vascular diseases independent of angiography duration and X-ray, however, IFN-γ has no effects in the Iranian patients suffering from cardiovascular diseases.
Interley̆kin-10 (IL-10) ta interferon-gamma (IFN-γ) ie vazhlyvymy, vidpovidno, proty- ta prozapal\'nymy tsy- tokinamy, iaki mozhut\' braty uchast\' u patogenezi sertsevo-sudynnykh zakhvoriuvan\'. Krim togo, faktory navkolysh- n\'ogo seredovyshcha – taki, iak rentgenivs\'ke vyprominiuvannia – zdatni moduliuvaty ekspresiiu tsytokiniv. Cherez te, shcho rentgenivs\'ke vyprominiuvannia vykorystovuiet\'sia pry angiografiï, tsia protsedura, mozhlyvo, zminiuie ekspresiiu tsytokiniv.Meta. Otsinyty rivni IL-10 ta IFN-γ syrovatky krovi u patsiientiv iz sertsevo-sudynnymy zakhvoriuvanniamy (u iakykh naiavnyy̆ abo zh vidsutniy̆ stenoz sudyn) porivniano zi zdorovymy kontroliamy, a takozh efekty angiografiï shchodo vmistu tsytokiniv u syrovattsi krovi.Materialy ta metody. Doslidzhennia bulo provedeno sered 80 uchasnykiv – po 20 vypadkiv u kozhniy̆ grupi (zdo- rovi kontroli ta osoby iz sertsevo-sudynnymy zakhvoriuvanniamy bez stenozu sudyn, iz stenozom 1 sudyny ta ste- nozom bil\'sh nizh 1 sudyny) dlia otsinky rivniv IL-10 ta IFN-γ u syrovattsi krovi za dopomogoiu metodu imunofer- mentnogo analizu. Dlia z’iasuvannia vplyvu protsedury angiografiï na riven\' IL-10 ta IFN-γ u syrovattsi krovi, vmist obokh tsytokiniv takozh porivniuvaly u grupakh 2, 3 ta 4 do ta pislia angiografichnogo doslidzhennia. Rezul\'taty. Riven\' IL-10, ale ne IFN-γ, u syrovattsi krovi zdorovykh osib grupy kontroliu buv bil\'shym, nizh u vsikh patsiientiv iz sertsevo-sudynnymy zakhvoriuvanniamy. Riven\' IL-10 ta IFN-γ u syrovattsi krovi ne zminiuvavsia pislia angiografiï, a takozh ne vidrizniavsia u kurtsiv ta osib, iaki ne kuriat\', tak samo iak i u spozhyvachiv opiiu ta osib, iaki opiy̆ ne vzhyvaiut\'.Vysnovky. Zavdiaky rezul\'tatam mozhna zrobyty vysnovok, shcho IL-10 mozhna y̆movirno vvazhaty ingibitorom ser- tsevo-sudynnykh zakhvoriuvan\', nezalezhno vid tryvalosti angiografiï ta rentgenografiï, v toy̆ chas iak IFN-γ ne pro- iavliaie efektiv u irans\'kykh patsiientiv, iaki strazhdaiut\' na sertsevo-sudynni zakhvoriuvannia.