背景:蜱携带微生物,其中一些对人类和动物有致病性。为了评估这一单一健康挑战,作为欧洲项目“保护我们的未来”的一部分,在芬兰的10家兽医诊所从宠物狗和猫身上收集了342只蜱虫。
方法:确定了蜱种,用定量PCR(qPCR)筛选蜱传病原体,包括伯氏疏螺旋体,miyamotoiBorreliamiyamotoi,犬埃里希亚,支原体属。,米库伦念珠菌,蜱传脑炎病毒(TBEV),和Babesiaspp.为了比较,用16S下一代测序(NGS)分析了一部分蜱DNA(20份qPCR阳性样本).
结果:大多数蜱是蓖麻(289,84.5%),其次是横齿肌(51,14.9%)。一个混合滴答(I.蓖麻/I.persulcatus,0.3%),并鉴定出一个血根虫蜱(0.3%)。我们在17%(59/342)的蜱中发现了一种或多种分析的病原体。最普遍的病原体是B.burgdorferis.l.(36,10.5%),其次是吞噬体(12,3.5%),B.miyamotoi(5,1.5%),维纳巴贝虫(4,1.2%),和TBEV(1,0.3%)。从三个(0.9%)蜱中扩增出念珠菌DNA。未检测到犬埃里希氏菌。在16SNGS中,六个样品产生足够的读数用于分析。在这六个样本中,我们证实了疏螺旋体属的所有阳性qPCR结果。Ca。N.米库伦西斯。
结论:本研究蜱中病原微生物的高患病率强调了对蜱和蜱传播疾病的认识和预防的重要性。此外,结果表明,兽医监测可以促进早期发现蜱传病原体和新的蜱种,并提请注意可能的共感染,应考虑在蜱叮咬后有症状的人和动物。
BACKGROUND: Ticks carry microbes, some of which are pathogenic for humans and animals. To assess this One Health challenge, 342 ticks were collected from pet dogs and cats at 10 veterinary clinics in Finland as part of the European project \"Protect Our Future Too\".
METHODS: The tick species were identified, and ticks were screened with quantitative PCR (qPCR) for tick-borne pathogens, including Borrelia burgdorferi sensu lato, Borrelia miyamotoi, Ehrlichia canis, Anaplasma spp., Candidatus Neoehrlichia mikurensis, tick-borne encephalitis virus (TBEV), and Babesia spp. For comparison, a subset of tick DNA (20 qPCR-positive samples) was analysed with 16S next-generation sequencing (NGS).
RESULTS: Most ticks were Ixodes ricinus (289, 84.5%), followed by Ixodes persulcatus (51, 14.9%). One hybrid tick (I. ricinus/I. persulcatus, 0.3%) and one Rhipicephalus sanguineus tick (0.3%) were identified. We found one or more of the analysed pathogens in 17% (59/342) of the ticks. The most prevalent pathogen was B. burgdorferi s.l. (36, 10.5%), followed by Anaplasma phagocytophilum (12, 3.5%), B. miyamotoi (5, 1.5%), Babesia venatorum (4, 1.2%), and TBEV (1, 0.3%). Candidatus Neoehrlichia mikurensis DNA was amplified from three (0.9%) ticks. Ehrlichia canis was not detected. In the 16S NGS, six samples produced enough reads for the analysis. In these six samples, we confirmed all the positive qPCR findings of Borrelia spp. and Ca. N. mikurensis.
CONCLUSIONS: The high prevalence of pathogenic microorganisms in the ticks of this study emphasizes the importance of awareness of ticks and tick-borne diseases and prevention. Furthermore, the results show that veterinary surveillance can facilitate early detection of tick-borne pathogens and new tick species and draw attention to possible co-infections that should be considered both in symptomatic humans and animals after tick bites.