铅影响光合作用和生长,对植物有严重的毒性作用。这里,在醋酸铅溶液的不同应用下,研究了D.huoshanense的差异表达蛋白(DEPs)。使用无标记定量蛋白质组学方法,超过12,000个肽和2,449个蛋白质被鉴定。GO和KEGG功能注释表明,这些差异蛋白主要参与碳水化合物代谢,能量代谢,氨基酸代谢,翻译,蛋白质折叠,排序,和退化,以及氧化和还原过程。总共确定了636个DEP,铅可以诱导大多数蛋白质的表达。KEGG富集分析表明,参与同源重组等过程的蛋白质,维生素B6代谢,类黄酮生物合成,细胞成分组织或生物发生,和生物调控显著丰富。近40种蛋白质参与DNA复制和修复,RNA合成,运输,和拼接。铅胁迫对霍山D的影响可能通过光合作用来实现,氧化磷酸化,和产生过量的抗氧化物质。铅胁迫后9种光合作用相关蛋白和12种氧化磷酸化相关蛋白的表达上调。此外,总共3个SOD,12POD,3CAT,并鉴定出7种抗坏血酸相关代谢酶。在铅应力下,几乎所有参与抗氧化物质合成的关键酶都被上调,这可以促进氧自由基的清除。参与糖和糖苷合成的一些关键酶的表达水平,苯丙素合成途径,萜烯合成途径也有所增加。还鉴定了30多种参与重金属运输的蛋白质。表达谱分析显示ABC型多药耐药转运蛋白的表达显着上升,铜伴侣,和暴露于铅胁迫的P型ATP酶。本研究结果为研究霍山毛虫对重金属胁迫的响应和抗性奠定了基础。
Lead affects photosynthesis and growth and has serious toxic effects on plants. Here, the differential expressed proteins (DEPs) in D. huoshanense were investigated under different applications of lead acetate solutions. Using label-free quantitative proteomics methods, more than 12,000 peptides and 2,449 proteins were identified. GO and KEGG functional annotations show that these differential proteins mainly participate in carbohydrate metabolism, energy metabolism, amino acid metabolism, translation, protein folding, sorting, and degradation, as well as oxidation and reduction processes. A total of 636 DEPs were identified, and lead could induce the expression of most proteins. KEGG enrichment analysis suggested that proteins involved in processes such as homologous recombination, vitamin B6 metabolism, flavonoid biosynthesis, cellular component organisation or biogenesis, and biological regulation were significantly enriched. Nearly 40 proteins are involved in DNA replication and repair, RNA synthesis, transport, and splicing. The effect of lead stress on D. huoshanense may be achieved through photosynthesis, oxidative phosphorylation, and the production of excess antioxidant substances. The expression of 9 photosynthesis-related proteins and 12 oxidative phosphorylation-related proteins was up-regulated after lead stress. Furthermore, a total of 3 SOD, 12 POD, 3 CAT, and 7 ascorbate-related metabolic enzymes were identified. Under lead stress, almost all key enzymes involved in the synthesis of antioxidant substances are up-regulated, which may facilitate the scavenging of oxygen-free radical scavenging. The expression levels of some key enzymes involved in sugar and glycoside synthesis, the phenylpropanoid synthesis pathway, and the terpene synthesis pathway also increased. More than 30 proteins involved in heavy metal transport were also identified. Expression profiling revealed a significant rise in the expression of the ABC-type multidrug resistance transporter, copper chaperone, and P-type ATPase with exposure to lead stress. Our findings lay the basis for research on the response and resistance of D. huoshanense to heavy metal stress.