水山药的细菌多样性和组成(DioscoreaalataL.cv。A-19),它可以在没有化学肥料的情况下生长,最近的特点是与使用化学施肥相比没有显着差异。然而,与白山药(Dioscorearotundata)相关的细菌的多样性和群落结构,西非种植最多的和经济上最重要的山药,尚未被调查。这项研究表征了与散装土壤相关的细菌多样性和组成,根际,在伊巴丹的田间试验中,六种白几内亚山药基因型(S004,S020,S032,S042,S058和S074)的植物根,尼日利亚施用氮基化肥。在大块土壤中发现了最大的细菌多样性,其次是根际和根。根据阿尔法多样性分析,在散装土壤样品中,S020和S042中的细菌多样性都随着肥料的施用而增加。在无肥条件下生长的S058在根际样品中具有最高的细菌多样性。β多样性分析强调了与基因型和肥料处理相关的细菌组成的显着差异,与其他基因型相比,S032具有独特的细菌组成。所有样品类型的优势门是变形杆菌。放线菌是散装土壤样品中的优势门。在属一级,芽孢杆菌是对照和处理样品中最丰富的细菌属。假单胞菌在所有根际样品中占主导地位。金杆菌,鞘氨醇,根据基因型,与根际相关的Delftia和Klebsiella显示对照样品和处理样品之间的相对丰度发生变化。一个与共生固氮菌有关的属,变种根瘤菌-新根瘤菌-副根瘤菌-根瘤菌进化枝,在所有根样中显示出较高的相对丰度,表明它是核心细菌属。此外,化肥的田间施用对与共生固氮剂相关的两个属的相对丰度有重大影响,根际和根中的根际根瘤菌-新根瘤菌-根瘤菌-根瘤菌进化枝和缓生根瘤菌。这些结果表明,氮基化肥和植物基因型会影响相关细菌群落的组成排列,包括共生固氮菌.
The bacterial diversity and composition of water yam (Dioscorea alata L. cv. A-19), which can grow without chemical fertilization, have recently been characterized with no significant differences compared with the use of chemical fertilization. However, the diversity and community structure of bacteria associated with the white Guinea yam (Dioscorea rotundata), the most cultivated and economically important yam in West Africa, have not yet been investigated. This study characterized the bacterial diversity and composition associated with bulk soil, rhizosphere, and plant roots in six white Guinea yam genotypes (S004, S020, S032, S042, S058, and S074) in field experiments in Ibadan, Nigeria under N-based chemical fertilizer application. The largest diversity of bacteria was found in the bulk soil, followed by the rhizosphere and roots. Based on the alpha diversity analysis, the bacterial diversity in both S020 and S042 increased with fertilizer application among the bulk soil samples. S058 grown under no-fertilizer conditions had the highest bacterial diversity among the rhizosphere samples. Beta diversity analysis highlighted the significant difference in the composition of bacteria associated with the genotypes and fertilizer treatments, and S032 had a unique bacterial composition compared to the other genotypes. The dominant phylum across all sample types was Proteobacteria. Actinobacteriota was the dominant phylum among bulk soil samples. At the genus level, Bacillus was the most abundant bacterial genus across both the control and treated samples. Pseudomonas was predominant across all rhizosphere samples. Chryseobacterium, Sphingobium, Delftia and Klebsiella associated with the rhizosphere were shown the altered relative abundance between the control and treated samples depending on genotypes. A genus related to symbiotic nitrogen-fixing bacteria, the Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium clade, showed higher relative abundance among all root samples, indicating that it is a core bacterial genus. Furthermore, the field application of chemical fertilizer had a significant impact on the relative abundances of two genera related to symbiotic nitrogen-fixers, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium clade and Bradyrhizobium in the rhizosphere and root. These results suggest that N-based chemical fertilizers and plant genotypes would influence the compositional arrangement of associated bacterial communities, including symbiotic nitrogen-fixing bacteria.