库拉索芦荟长期以来一直被用作食品和药品。为了研究巴布拉的化学成分及其对磷酸二酯酶-4D(PDE4D)的抑制活性,使用干燥的A.barbadensis粉末的70%甲醇提取物。植物化学研究导致了三种新的色原的分离,5-(羟甲基)-7-甲氧基-2-甲基色酮(4),5-((4E)-2'-氧代-戊烯基)-2-羟甲基色酮(6),和7-羟基-5-(羟甲基)-2-甲基色酮(7),连同18种已知化合物。它们的化学结构是根据光谱方法确定的,包括UV,IR,1D和2DNMR,和HRMS光谱法。此外,它们对PDE4D的抑制作用使用氚标记的腺苷3'进行评估,5'-环状单磷酸酯((3)H-cAMP)作为底物。通过反应后放射性的变化来计算抑制作用,化合物1-4、10和21对PDE4D表现出一定的抑制活性,这可以解释为什么A.barbadensis可以作为抗炎剂。
Aloe barbadensis Mill has been used as food and medicine for a long time. In order to investigate the chemical constituents of A. barbadensis and their inhibitory activities towards phosphodiesterase-4D (PDE4D), 70% methanol extract of the dried A. barbadensis powder was employed. Phytochemical investigation has led to the isolation of three new chromones, 5-(hydroxymethyl)-7-methoxy-2-methylchromone (4), 5-((4E)-2\'-oxo-pentenyl)-2-hydroxymethylchromone (6), and 7-hydroxy-5-(hydroxymethyl)-2-methylchromone (7), together with eighteen known compounds. Their chemical structures were determined based on spectroscopic methods including UV, IR, 1D and 2D NMR, and HRMS spectrometry. In addition, their inhibition against PDE4D was evaluated using tritium-labeled adenosine 3\',5\'-cyclic monophosphate ((3)H-cAMP) as the substrate. Inhibition was calculated by the variation of radioactivity after the reaction, and compounds 1-4, 10, and 21 exhibited certain inhibitory activities towards PDE4D, which can provide an explanation why A. barbadensis can serve as anti-inflammatory agents.