沿海木麻黄是海南沿海防护林中最常见的树种。对其化感内生菌的基因组进行测序可以使这些细菌的保护作用在受保护的森林中得到有效实施。这项研究的目的是对从木叶梭菌根组织中分离的内生菌解淀粉芽孢杆菌和芽孢杆菌的全基因组进行测序。结果表明,解淀粉芽孢杆菌和阿里亚巴泰的基因组大小分别为3.854Mb和5.508Mb,分别。这两个菌株共有2514个共同基因家族,同时具有1055和2406个不同的基因家族,分别。这两个菌株具有283和298个化感化学合成相关基因,分别,其中255个是两个菌株共有的,其中28个和43个是每个菌株独有的,分别。这些基因被推定参与11个功能通路,包括次级代谢产物的生物合成,萜烯碳骨架生物合成,泛醌和其他萜烯醌的生物合成,托烷/哌啶和哌啶生物碱生物合成,和苯丙素生物合成。已知NQO1和entC参与泛醌和其他萜类醌的生物合成,和rfbc/rmlC,rfbA/rmlA/rffH,和rfbB/rmlB/rffG参与聚酮聚糖单元的生物合成。在B.aryabhattai特异性化感化学合成相关基因中,STE24参与萜烯碳骨架的生产,atzF和gdhA在精氨酸生物合成中的作用,和TYR在异喹啉生物碱生物合成中的作用。解淀粉芽孢杆菌和B.aryabhattai共享aspB基因,yhdR,trpA,trpB,还有GGPS,已知参与类胡萝卜素的合成,吲哚,莫米拉内酯,和其他化感物质。此外,这些细菌通过聚酮糖单元生物合成和异喹啉生物碱生物合成等途径参与化感化学合成。本研究揭示了与木薯芽孢杆菌相关的化感作用的遗传基础。强调这些细菌在杂草管理和作物保护的可持续农业战略中的可能用途。
The coastal Casuarina equisetifolia is the most common tree species in Hainan\'s coastal protection forests. Sequencing the genomes of its allelopathic endophytes can allow the protective effects of these bacteria to be effectively implemented in protected forests. The goal of this study was to sequence the whole genomes of the endophytes Bacillus amyloliquefaciens and Bacillus aryabhattai isolated from C. equisetifolia root tissues. The results showed that the genome sizes of B. amyloliquefaciens and B. aryabhattai were 3.854 Mb and 5.508 Mb, respectively. The two strains shared 2514 common gene families while having 1055 and 2406 distinct gene families, respectively. The two strains had 283 and 298 allelochemical synthesis-associated genes, respectively, 255 of which were shared by both strains and 28 and 43 of which were unique to each strain, respectively. The genes were putatively involved in 11 functional pathways, including secondary metabolite biosynthesis, terpene carbon skeleton biosynthesis, biosynthesis of ubiquinone and other terpene quinones, tropane/piperidine and piperidine alkaloids biosynthesis, and phenylpropanoid biosynthesis. NQO1 and entC are known to be involved in the biosynthesis of ubiquinone and other terpenoid quinones, and rfbC/rmlC, rfbA/rmlA/rffH, and rfbB/rmlB/rffG are involved in the biosynthesis of polyketide glycan units. Among the B. aryabhattai-specific allelochemical synthesis-related genes, STE24 is involved in terpene carbon skeleton production, atzF and gdhA in arginine biosynthesis, and TYR in isoquinoline alkaloid biosynthesis. B. amyloliquefaciens and B. aryabhattai share the genes aspB, yhdR, trpA, trpB, and GGPS, which are known to be involved in the synthesis of carotenoids, indole, momilactones, and other allelochemicals. Additionally, these bacteria are involved in allelochemical synthesis via routes such as polyketide sugar unit biosynthesis and isoquinoline alkaloid biosynthesis. This study sheds light on the genetic basis of
allelopathy in Bacillus strains associated with C. equisetifolia, highlighting the possible use of these bacteria in sustainable agricultural strategies for weed management and crop protection.