黄曲霉毒素B1(AFB1)的食品污染是全球关注的问题,对动物和人类健康产生不利影响。该研究旨在评估α硫辛酸(ALA)和/或辅酶Q10(CQ10)对AFB1对肝脏和肾脏的有害影响的保护作用。将56只成年雄性Wistar白化病大鼠(180-200g)分为7组,每个有八只大鼠:(1)给予生理盐水作为对照,(2)ALA(100mg/kgbw/day)通过胃管饲法给予15天,(3)通过胃管饲法给予CQ10(10mg/kgbw/day)15天。组(4)在第12天和第14天口服AFB1(2.5mg/kgbw),(5)接受AFB1和ALA,(6)收到AFB1和CQ10,(7)收到AFB1,ALA,和CQ10,如先前在ALA中所述,CQ10和AFB1组。暴露于AFB1后,肝脏标志物显著增加(AST,ALT,ALP,与对照组相比,观察到LDH)和肾功能检查(BUN和肌酐)。ALA和/或CQ10显着降低肝脏和肾脏功能的酶,与AFB1相比。AFB1暴露破坏了氧化剂和抗氧化剂之间的平衡。尽管如此,ALA和/或CQ10引起的氧化应激(MDA,NO,和8-OHdG)低得多和抗氧化活性(GSH,GSH-Px,SOD,和CAT)更高。当我们一起使用两者时,活动符合控制水平。有趣的是,这项研究表明,ALA和CQ10显着降低IL-1β,当在AFB1暴露后一起使用时,IL-6和TNF-α水平与对照值相比引起强烈炎症。一些CQ10治疗参数显著优于ALA。在减少炎症方面,ALA和CQ10一起比单独的任何一种都更好地防止AFB1在肝和肾实质中引起的毒性。防止DNA损伤,与自由基作斗争。
Food contamination with Aflatoxin B1 (AFB1) is a worldwide concern that adversely affects animal and human health. The study aimed to evaluate the protective effect of alpha lipoic acid (
ALA) and/or co-enzyme Q10 (CQ10) against the harmful effects of AFB1 on the liver and kidneys. Fifty-six mature male Wistar Albino rats (180-200 g) were divided into seven groups, each with eight rats: (1) saline was given as a control, (2)
ALA (100 mg/kg bw/day) was given by stomach gavage for fifteen days, and (3) CQ10 (10 mg/kg bw/day) was given by stomach gavage for fifteen days. Group (4) orally given AFB1 (2.5 mg/kg bw) on days 12th and 14th, (5) received AFB1 and ALA, (6) received AFB1 and CQ10, and (7) received AFB1,
ALA, and CQ10, as previously described in the
ALA, CQ10, and AFB1 groups. After the exposure to AFB1, a significant increase in liver markers (AST, ALT, ALP, and LDH) and renal function tests (BUN and creatinine) was observed compared with the control.
ALA and/or CQ10 significantly reduced enzymes of liver and renal functions, as compared with AFB1. AFB1 exposure threw off the balance between oxidants and antioxidants. Still, ALA and/or CQ10 made oxidative stress (MDA, NO, and 8-OHdG) much lower and antioxidant activities (GSH, GSH-Px, SOD, and CAT) much higher. When we used the two together, the activities matched the control levels. Interestingly, this study shows that ALA and CQ10 significantly lowered IL-1β, IL-6, and TNF-α levels compared to the control values when used together after AFB1 exposure caused robust inflammation. Some CQ10 treatment parameters significantly outperformed those of
ALA. ALA and CQ10 together worked better than either one alone to protect against AFB1-induced toxicity in the hepatic and renal parenchyma in terms of reducing inflammation, preventing DNA damage, and fighting free radicals.