Akabane病毒(AKAV)是一种昆虫传播的病毒,属于Perbunyaviridae科的直鼻病毒属。它是Akabane病(AD)的病因,出现在亚洲,澳大利亚,以及中东给家畜和野生动物造成了严重的经济损失。AKAV在埃及没有得到足够的重视,它在埃及动物中的证据从未被报道过。因此,这项研究使用ELISA测定法来调查AKAV在Beheira省八个地区的埃及奶牛和肉牛中的血清阳性率,北埃及。在368份调查的血浆样本中,总体AKAV血清阳性率为54.3%(95%CI:50.8~61.4).在所有检查的牛场(7/7)和大多数屠宰场(8/9)中检测到AKAV抗体。年龄,性别,品种,和检测牛的位置被分析为AKAV感染的危险因素。在>5岁的牛中获得了血清阳性的更高的显着增加(p<0.0001;OR=9.4),女性(p<0.0001,OR=8.3),或Holstein品种(p<0.0001,OR=22.6)比年轻的年龄,男性,以及混合和哥伦比亚齐布品种,分别。此外,我们发现,在受试地点之间,AKAV血清阳性率存在显著差异.最终,多变量分析得出年龄(p=0.002,OR=3.32,95%CI=1.57-7.04)和品种(p=0.03,OR=1.69,95%CI=1.05-2.72)是AKAV感染的显著风险.总之,这项研究是首次在埃及动物中检测AKAV感染。
Akabane virus (AKAV) is an insect-borne virus belonging to the genus Orthobunyavirus of the family Peribunyaviridae. It is the etiologic agent of Akabane disease (AD), which emerged in Asia, Australia, and the Middle East causing severe economic losses among domestic and wild animals. AKAV has not received enough attention in Egypt, and its evidence among Egyptian animals has never been reported. Therefore, this study used ELISA assay to investigate the seroprevalence of AKAV among Egyptian dairy and beef cattle in eight localities of Beheira province, north Egypt. Out of 368 investigated plasma samples, the overall AKAV seroprevalence was 54.3% (95% CI: 50.8-61.4). AKAV antibodies were detected in all examined cattle farms (7/7) and the majority of abattoirs (8/9). Age, sex, breed, and location of the tested cattle were analyzed as risk factors for AKAV infection. A higher significant increase in seropositivity was obtained in cattle who were aged >5 years (p < 0.0001; OR = 9.4), females (p < 0.0001, OR = 8.3), or Holstein breed (p < 0.0001, OR = 22.6) than in younger ages, males, and Mixed and Colombian zebu breeds, respectively. Moreover, a significant variation in AKAV seroprevalence between the tested locations was noticed. Ultimately, a multivariable analysis concluded that age (p = 0.002, OR = 3.32, 95% CI = 1.57-7.04) and breed (p = 0.03, OR = 1.69, 95% CI = 1.05-2.72) were significant risks for AKAV infection. In conclusion, this study is the first to detect AKAV infection in Egyptian animals.