背景:衰老导致骨骼肌的丧失,肌肉力量减弱,身体机能下降。
目的:本研究评估了Withaniasomnifera和一些饮食干预措施,以对抗衰老大鼠的肌肉无力。
方法:将对应于60-65岁人类年龄的大鼠(12-13个月大)分配到各个组,并口服标准的睡梦草提取物(WSE,500mg/kg)或包含大豆(1.5g/kg)和藜麦(1g/kg)的蛋白质混合物或WSE和蛋白质混合物或乳清蛋白(1g/kg)的组合作为参考标准或仅抗阻运动60天。每周监测握力和血糖水平。在治疗结束时,总蛋白质,炎症标志物(CRP,IL-6和TNF-α),AMPK,丙二醛,谷胱甘肽,测定抗氧化酶和凋亡调节基因(Bax和Bcl-2)。对所有动物的肱二头肌进行组织形态学研究。
结果:所有治疗均成功减轻了衰老升高的葡萄糖,CRP,IL-6,TNF-α,AMPK,丙二醛,和Bax水平。显着恢复老化耗尽的总蛋白质水平,谷胱甘肽,超氧化物歧化酶,过氧化氢酶,在治疗组中观察到Bcl-2。所有治疗的握力增加和肱二头肌质量增加表明衰老肌肉的力量和功能恢复。WSE+蛋白质处理在所有处理组中引起最佳结果以优化肌肉力量。
结论:所有干预措施都通过减少炎症来抑制肌肉损失并增强骨骼肌,氧化应激和细胞凋亡,并增加肌肉的ATP可用性。
BACKGROUND: Aging leads to loss of skeletal muscle, diminished muscle strength, and decline in physical functions.
OBJECTIVE: This study evaluates Withania somnifera and some dietary interventions to combat muscle weakness in aging rats.
METHODS: Rats (12-13 months old) corresponding to a human age of 60-65 years were assigned to various groups and given orally a standardized W. somnifera extract (WSE, 500 mg/kg) or a protein cocktail comprising soybean (1.5 g/kg) and quinoa (1 g/kg) or a combination of WSE and the protein cocktail or whey protein (1 g/kg) as a reference standard or only resistance exercise for 60 days. Grip strength and blood glucose levels were monitored weekly. At the end of the treatment, total protein, inflammatory markers (CRP, IL-6 and TNF-α), AMPK, malondialdehyde, glutathione, antioxidant enzymes and apoptotic regulator genes (Bax and Bcl-2) were assayed. The biceps brachii muscle of all animals was subjected to histomorphological study.
RESULTS: All treatments successfully attenuated aging-elevated glucose, CRP, IL-6, TNF-α, AMPK, malondialdehyde, and Bax levels. A significant restoration of the aging-depleted total protein levels, glutathione, superoxide dismutase, catalase, and Bcl-2 was noted in the treatment groups. An increase in grip strength and greater biceps mass with all treatments indicated regaining of the frail aging muscle\'s strength and functionality. The WSE + protein treatment elicited the best results among all treatment groups to optimize muscle strength.
CONCLUSIONS: All the interventions curbed muscle loss and strengthened the skeletal muscle by reducing inflammation, oxidative stress and apoptosis, and increasing ATP availability to the muscle.