在这项研究中,研究了海棠甲醇提取物的植物化学特征和纳米银(AgNP)合成能力。此外,AgNPs的生物应用,如抗菌作用(对肺炎克雷伯菌,金黄色葡萄球菌,枯草芽孢杆菌,和大肠杆菌),蚊子杀虫作用(对斯蒂芬氏按蚊,库蚊,和埃及伊蚊),使用体外实验评估细胞毒性(针对成纤维细胞细胞系L929)。植物化学分析显示甲醇提取物含有强心苷,萜类化合物,皂苷,生物碱,黄酮类化合物,糖苷,香豆素,酚类物质,还有单宁.此外,标准表征技术,如紫外-可见光谱法,SEM,TEM,FTIR,XRD和XRD证实了绿脓杆菌的甲醇提取物有效地合成了AgNPs。合成的AgNP具有球形形状和20-200nm的尺寸。杀菌分析显示AgNP具有剂量依赖性抗菌活性。MTT分析表明,在正常成纤维细胞系L929中,AgNPs的生物相容性高达250μg·mL-1。此外,AgNPs对An幼虫的LC50值。Stephensi,Cx.Quinquefasciatus,还有Ae.埃及伊蚊分别为0.4、4.7和1.2ppm,分别。田间试验表明,杀幼虫作用在24-72小时内增强,减少的速度随着时间的推移而增加。因此,我们的发现提供了一种理想的可持续AgNP生物农药来对抗丝虫,登革热,和疟疾病媒。
In this study, the phytochemical profile and silver nanoparticle (
AgNPs)-synthesizing ability of Pittosporum undulatum methanol extract were investigated. Furthermore, biological applications of the
AgNPs, such as antibacterial effect (against Klebsiella pneumoniae, Staphylococcus aureus, Bacillus subtilis, and Escherichia coli), mosquito larvicidal effect (against Anopheles stephensi, Culex quinquefasciatus, and Aedes aegypti), and cytotoxicity (against fibroblast cell line L929) were evaluated using in vitro experiments. The phytochemical analysis revealed that the methanol extract contained cardiac glycosides, terpenoids, saponins, alkaloids, flavonoids, glycosides, coumarins, phenolics, and tannins. Furthermore, standard characterization techniques such as UV-Vis spectrometry, SEM, TEM, FTIR, and XRD confirmed that the methanol extract of P. undulatum effectively synthesized the AgNPs. The synthesized
AgNPs had a spherical shape and size of 20-200 nm. The bactericidal analysis revealed that the
AgNPs have dose-dependent antibacterial activity. The MTT assay showed that the AgNPs were bio-compatible up to a dosage of 250 μg mL-1 in the normal fibroblast cell line L929. Furthermore, the LC50 values for
AgNPs against larvae of An. stephensi, Cx. quinquefasciatus, and Ae. aegypti were 0.4, 4.7, and 1.2 ppm, respectively. Field trials demonstrated that the larvicidal effect was enhanced within 24-72 h, and the rate of reduction increased over time. Thus, our findings provide an ideal sustainable AgNP bio-pesticide to combat filarial, dengue, and malaria vectors.