非孢子形成,革兰氏染色阳性,短杆状应变,指定SJQ22T,是从上海收集的水稻土样品中分离出来的,公关中国。对16SrRNA基因序列的比较分析表明,菌株SJQ22T属于Aerococcus属,与草绿色气溶胶(98.6%序列相似性)和乌氏气溶胶(98.5%序列相似性)类型菌株形成清晰的簇。菌株SJQ22T在30-45°C生长(最佳,30°C),pH6.0-8.0(最佳,pH7.0)和NaCl浓度为0-4%(最佳,1%)。细胞对氧化酶和过氧化氢酶活性呈阴性。化学分类学分析表明,菌株SJQ22T具有C16:0和C18:1ω9c作为主要脂肪酸。DNAG+C含量为39.0mol%。菌株SJQ22T与A.viridansATCC11563T的DNA-DNA相关性水平为13±2%,与A.urinaequiIFO12173T的DNA-DNA相关性水平为9±2%。根据获得的数据,菌株SJQ22T代表了一种新型的空气球菌属,其名称为Aerococcusagrisolisp.11月。是提议的。菌株类型为SJQ22T(=JCM33111T=CCTCCAB2018283T)。
A non-spore-forming, Gram-stain-positive, short rod-shaped strain, designated SJQ22T, was isolated from a paddy soil sample collected in Shanghai, PR China. A comparative analysis of 16S rRNA gene sequences showed that strain SJQ22T fell within the genus
Aerococcus, forming a clear cluster with the type strains of
Aerococcus viridans (98.6 % sequence similarity) and
Aerococcus urinaeequi (98.5 % sequence similarity). Strain SJQ22T grew at 30-45 °C (optimum, 30 °C), pH 6.0-8.0 (optimum, pH 7.0) and with a NaCl concentration of 0-4 % (optimum, 1 %). Cells were negative for oxidase and catalase activity. Chemotaxonomic analysis showed that strain SJQ22T possessed C16:0 and C18:1 ω9c as the predominant fatty acids. The DNA G + C content was 39.0 mol%. Strain SJQ22T exhibited DNA-DNA relatedness levels of 13±2 % with A. viridans ATCC 11563T and 9±2 % with A. urinaeequi IFO 12173T. Based on the data obtained, strain SJQ22T represents a novel species of the genus
Aerococcus, for which the name
Aerococcus agrisoli sp. nov. is proposed. The type strain is SJQ22T (=JCM 33111T=CCTCC AB 2018283T).