在这项研究中,金纳米粒子(AuNPs)从刺梨生物还原,一种以治疗各种疾病而闻名的药草。使用植物提取物的不同部分,包括甲醇馏分(ABMF),正己烷馏分(ABHF),氯仿馏分(ABCF),和用于AuNPs合成的水提取物。AuNPs的表征使用紫外-可见分光光度法进行,FT-IR,XRD,EDX,和TEM。紫外-可见光谱证实了AuNP的形成,在555nm处观察到峰。FT-IR分析表明植物化学物质在AuNPs表面有很强的覆盖作用,这得到了AuNPs中较高的总酚含量(TPC)和总黄酮含量(TFC)的支持。XRD结果表明,AuNPs的结晶度较高,粒径分布较小。TEM分析显示AuNP的球形,平均尺寸为29±10nm。生物合成的AuNPs表现出优异的抗菌性能,抗氧化剂,和与植物提取物部分相比的细胞毒性活性。a.bracteosa中存在活性生物分子,比如neoclerodan黄酮醇苷,二萜,植物蜕皮激素,和环烯醚萜苷,有助于增强AuNPs的生物活性。总的来说,这项研究强调了A.bracteosa衍生的AuNP在各种生物医学应用中的潜力,因为它们具有显着的治疗特性和有效的植物化学物质封顶。研究重点:这项研究通过探索Ajugabracteosa和金纳米颗粒(AuNPs)的治疗潜力,强调了草药在当代医疗保健中日益增长的意义。该研究强调了A.bracteosa叶提取物和AuNPs在治疗细菌感染方面的显着功效,证明了它们对一系列菌株的杀菌作用。通过爪水肿方法证明了植物提取物和纳米颗粒的抗炎特性,表明它们在控制炎症中的适用性。这些发现将A.bracteosa和AuNPs定位为现代药物替代和有效方法的潜在候选者。
In this study, gold nanoparticles (AuNPs) were bioreduced from Ajuga bracteosa, a medicinal herb known for its therapeutic properties against various diseases. Different fractions of the plant extract were used, including the methanolic fraction (ABMF), the n-hexane fraction (ABHF), the chloroform fraction (ABCF), and the aqueous extract for AuNPs synthesis. The characterization of AuNPs was performed using UV-Vis spectrophotometry, FT-IR, XRD, EDX, and TEM. UV-Vis spectroscopy confirmed the formation of AuNPs, with peaks observed at 555 nm. FT-IR analysis indicated strong capping of phytochemicals on the surface of AuNPs, which was supported by higher total phenolic contents (TPC) and total flavonoid contents (TFC) in AuNPs. XRD results showed high crystallinity and a smaller size distribution of AuNPs. TEM analysis revealed the spherical shape of AuNPs, with an average size of 29 ± 10 nm. The biologically synthesized AuNPs exhibited superior antibacterial, antioxidant, and cytotoxic activities compared to the plant extract fractions. The presence of active biomolecules in A. bracteosa, such as neoclerodan flavonol glycosides, diterpenoids, phytoecdysone, and iridoid glycosides, contributed to the enhanced biological activities of AuNPs. Overall, this research highlights the potential of A. bracteosa-derived AuNPs for various biomedical applications due to their remarkable therapeutic properties and effective capping by phytochemicals. RESEARCH HIGHLIGHTS: This research underscores the growing significance of herbal medicine in contemporary healthcare by exploring the therapeutic potential of Ajuga bracteosa and gold nanoparticles (AuNPs). The study highlights the notable efficacy of A. bracteosa leaf extracts and AuNPs in treating bacterial infections, demonstrating their bactericidal effects on a range of strains. The anti-inflammatory properties of plant extracts and nanoparticles are evidenced through paw edema method suggesting their applicability in managing inflammatory conditions. These findings position A. bracteosa and AuNPs as potential candidates for alternative and effective approaches to modern medication.