目的:从形态学上评估常规玻璃离聚物水泥(GIC)与牙本质之间的界面放置一天后,以及猴子牙齿老化/功能一年后界面的变化。
方法:在两只猴子的七颗完整牙齿的颊面上,准备了浅V类空洞,然后用富士IXGP(GC)填充以提供1年的体内数据。一年后,在体内1天组中,每只猴子的两颗牙齿同样制备和修复.第二天,拔除修复的牙齿,并使用透射电子显微镜(TEM)观察修复界面。此外,修复体同样放置在两个拔出的人类牙齿中(对照,1天体外组),并在放置后一天使用TEM进行观察。
结果:1天的体内和体外结果表明,GIC似乎通过类似于树脂粘合剂产生的杂化层的去矿质区与牙本质结合。然而,在体内1年后,GIC和牙本质之间的界面似乎随着时间的推移而改变:在再矿化层中和沿着胶原纤维检测到许多针状晶体.离子的缓慢扩散导致孔隙,充满了矿物晶体,使毛孔变小。
结论:GIC和牙本质之间的界面随着时间的推移而改变,并且在界面处可能发生重结晶或再矿化(体内1年)。
OBJECTIVE: To morphologically evaluate the interface between a conventional glass-ionomer cement (GIC) and dentin one day after placement, as well as the changes at the interface after one year of aging/functioning in monkey teeth.
METHODS: On the buccal surfaces of seven intact teeth in each of two monkeys, shallow class V cavities were prepared, which were then filled with Fuji IX GP (GC) to provide 1-year in vivo data. A year later, two more teeth in each monkey were similarly prepared and restored for the 1-day in vivo group. The following day, the restored teeth were extracted and the restoration interfaces observed using transmission electron microscopy (TEM). In addition, restorations were similarly placed in two extracted human teeth (control, 1-day in vitro group) and observed a day after placement using TEM.
RESULTS: The 1-day in vivo and in vitro results showed that the GIC appeared to bond to dentin through a demineralized zone similar to the hybrid layer produced by resinous adhesives. However, the interface between GIC and dentin after 1 year in vivo appeared to change over time: many needle-like crystals were detected within the remineralized layer and along the collagen fibrils. Slow diffusion of ions resulted in pores, which filled with mineral crystals and made the pores smaller.
CONCLUSIONS: The interface between GIC and dentin morphologically changes over time, and recrystallization or remineralization at the interface may occur (1 year in vivo).