■突触小泡的回收在小泡池补充中起着重要作用,神经递质释放和突触可塑性。Clathrin介导的内吞作用(CME)被认为是突触小泡补充的主要机制。AP-2(衔接子相关蛋白复合物2)和肌球蛋白Ⅵ是调控CME结构和动力学的关键蛋白。
■本研究旨在揭示AP-2/肌球蛋白Ⅵ在小鼠耳蜗内毛细胞(IHCs)中的时空表达及其与听觉功能的相关性。
■免疫荧光法检测AP-2和肌球蛋白Ⅵ在不同年龄CBA/CaJ小鼠耳蜗毛细胞中的定位和表达。采用qRT-PCR方法验证AP-2和肌球蛋白ⅥmRNA在小鼠耳蜗中的差异表达,对不同年龄的小鼠进行ABR试验。对AP-2/肌球蛋白Ⅵ水平与听觉功能的相关性进行了初步分析。
■AP-2位于IHCs的细胞质区域,主要在IHCs的基底区域和带状突触附近区域表达,而肌球蛋白Ⅵ在IHC和OHC的胞质区表达。此外,P7小鼠耳蜗中AP-2和肌球蛋白Ⅵ无明显表达,其表达水平在P35时达到峰值,然后随着年龄的增长而显著下降。小鼠耳蜗中AP-2和肌球蛋白Ⅵ的表达模式和表达水平与听觉系统的发育一致。
■AP-2和肌球蛋白Ⅵ蛋白的表达在不同年龄的小鼠中可能存在差异,这种变化可能导致CME效率的差异;它也可能导致IHC功能的缺陷。
UNASSIGNED: Recycling of synaptic vesicles plays an important role in vesicle pool replenishment, neurotransmitter release and synaptic plasticity. Clathrin-mediated endocytosis (CME) is considered to be the main mechanism for synaptic vesicle replenishment. AP-2 (adaptor-related protein complex 2) and myosin Ⅵ are known as key proteins that regulate the structure and dynamics of CME.
UNASSIGNED: This study aims to reveal the spatiotemporal expression of AP-2/myosin Ⅵ in inner hair cells (IHCs) of the mouse cochlea and its correlation with auditory function.
UNASSIGNED: Immunofluorescence was used to detect the localization and expression of AP-2 and myosin Ⅵ in cochlear hair cells (HCs) of CBA/CaJ mice of various ages. qRT-PCR was used to verify the differential expression of AP-2 and myosin Ⅵ mRNA in the mouse cochlea, and ABR tests were administered to mice of various ages. A preliminary analysis of the correlation between AP-2/myosin Ⅵ levels and auditory function was conducted.
UNASSIGNED: AP-2 was located in the cytoplasmic region of IHCs and was mainly expressed in the basal region of IHCs and the area near ribbon synapses, while myosin Ⅵ was expressed in the cytoplasmic region of IHCs and OHCs. Furthermore, AP-2 and myosin Ⅵ were not significant detected in the cochleae of P7 mice; the expression level reached a peak at P35 and then decreased significantly with age. The expression patterns and expression levels of AP-2 and myosin Ⅵ in the cochleae of the mice were consistent with the development of the auditory system.
UNASSIGNED: AP-2 and myosin Ⅵ protein expression may differ in mice of different ages, and this variation probably leads to a difference in the efficiency in CME; it may also cause a defect in IHC function.