生物分子的检测对于患者诊断至关重要,疾病管理,和许多其他应用程序。最近,已经探索了基于纳米和微粒的检测,通过减少所需的样品体积和测定时间以及增强可调性来改进传统测定。在这些方法中,将粒子运动与生物分子浓度耦合的基于主动粒子的测定通过简化的信号输出扩大测定的可及性。然而,这些方法中的大多数都需要二级标签,这会使工作流程复杂化并引入其他错误点。这里,我们展示了一个无标签的概念证明,使用电动活性粒子的基于运动的生物分子检测系统。我们准备了感应电荷电泳微传感器(ICEM),用于捕获两个模型生物分子,链霉亲和素和卵清蛋白,并且显示生物分子的特异性捕获导致通过浓度低至0.1nM的ICEM速度抑制的直接信号转导。这项工作为快速,简单,和使用活性颗粒的无标记生物分子检测。
Detection of biomolecules is essential for patient diagnosis, disease management, and numerous other applications. Recently, nano- and microparticle-based detection has been explored for improving traditional assays by reducing required sample volumes and assay times as well as enhancing tunability. Among these approaches, active particle-based assays that couple particle motion to biomolecule concentration expand assay accessibility through simplified signal outputs. However, most of these approaches require secondary labeling, which complicates workflows and introduces additional points of error. Here, we show a proof-of-concept for a label-free, motion-based biomolecule detection system using electrokinetic active particles. We prepare induced-charge electrophoretic microsensors (ICEMs) for the capture of two model biomolecules, streptavidin and ovalbumin, and show that the specific capture of the biomolecules leads to direct signal transduction through ICEM speed suppression at concentrations as low as 0.1 nM. This work lays the foundation for a new paradigm of rapid, simple, and label-free biomolecule detection using active particles.