体外研究了非电离毫米范围电磁波(MMEMW)(30-300GHz)对牛血清白蛋白(BSA)与吖啶橙(AO)相互作用特性的影响。选择频率41.8和50.3GHz,因为第一个是水的非共振频率,而第二个是水的共振。计算存在和不存在辐照时的结合常数和结合位点的数量。发现AO与BSA结合,而蛋白质照射后,相互作用力增强。然而,还表明,在41.8或50.3GHz辐照时,相互作用参数存在差异。AO与BSA结合,用频率为41.8GHz的MMEMW照射的频率要弱得多,除此之外,由MMEMW照射频率为50.3GHz。
该手稿致力于研究频率为41.8和50.3GHz的毫米波范围电磁波对模型生物系统的影响,处于组织的分子水平。目前,毫米波是环境电磁污染的重要组成部分,影响生物材料,除了这些波用于极高的频率治疗((30-300GHz),这是毫米范围的电磁波(1-10毫米))。另一方面,它们的作用机制问题主要与水有关,因为水分子的共振频率在毫米波的间隔内。在本研究中,作为这样的生物分子,已经选择了牛血清白蛋白,与吖啶橙相互作用。已知血清白蛋白携带和运输各种内源性和外源性试剂,包括整个循环系统的药物。反过来,吖啶橙已广泛用于生物染色,通过荧光发射来区分DNA和RNA,如今,它被认为是抗肿瘤治疗和诊断的有前途的药物。获得的数据表明,牛血清白蛋白与吖啶橙之间的相互作用发生变化,当白蛋白溶液被频率为41.8和50.3GHz的毫米波照射时。然而,相互作用的变化也取决于频率。因此,频率为41.8GHz的辐照会产生微不足道的变化,而频率为50.3GHz会引起测量参数的显着变化。这些研究是通过吸收进行的,荧光和CD光谱法。
The effect of non-ionizing millimeter range electromagnetic waves (MM EMW) (30-300 GHz) on the bovine serum albumin (BSA) interaction peculiarities with acridine orange (AO) has been studied in vitro. The frequencies 41.8 and 50.3 GHz were chosen, since the first one is nonresonant frequency for the water, while the second one is resonant for water. The binding constant and number of binding sites were calculated at both irradiation presence and absence. AO was revealed to bind to BSA, while after the protein irradiation the interaction force strengthens. However, it was also shown that there are differences of the interaction parameters while irradiating by 41.8 or 50.3 GHz. AO binds to BSA, irradiated by MM EMW with the frequency 41.8 GHz much more weaker, than to that, irradiated by MM EMW with the frequency 50.3 GHz.
The manuscript is devoted to the study of the effect of millimeter range electromagnetic waves with the frequencies 41.8 and 50.3 GHz on the model biological system, being on molecular level of organization. Nowadays millimeter range electromagnetic waves compose a significant part of electromagnetic pollution in the environment and affect biological material, besides these waves are used in extremely high frequencies-therapy ((30–300 GHz), which are millimeter range electromagnetic waves (1–10 mm)). On the other hand, the problem of their effect mechanism is mainly connected to water, since the resonant frequencies for water molecules are in the interval of millimeter waves. In the present study, as such biological molecule, the bovine serum albumin has been chosen, which interacts with acridine orange. Serum albumins are known to carry and transport various endogenous and exogenous agents, including drugs throughout circulatory system. In turn, acridine orange has been extensively used for biological staining to differentiate DNA from RNA by fluorescence emission for years, Nowadays, it is considered as a promising agent for antitumorous treatment and diagnosis.The data obtained show that the interaction between bovine serum albumin and acridine orange changes, when the solution of albumin is irradiated by the millimeter waves with the frequencies 41.8 and 50.3 GHz. However, the interaction alteration depends on the frequency as well. Thus, the irradiation with the frequency 41.8 GHz makes insignificant changes, while that with the frequency 50.3 GHz induces significant changes of measured parameters. The studies were conducted by absorption, fluorescence and CD spectroscopy methods.