菌株S30A2T,从蒙自铜矿酸性矿山排水沉积物中分离出来,云南,被提议代表硫氧化属酸性硫杆菌的一种新物种。细胞革兰氏染色阴性,非内生孢子形成,具有一个或两个单极鞭毛和杆状的高度能动。菌株是嗜温的,在30-50°C生长(最佳,38°C),嗜酸,在pH2.0-4.5下生长(最佳,pH2.5),并耐受0-4%(w/v;684moll-1)NaCl。基于16SrRNA基因的序列分析表明,菌株S30A2T属于嗜酸硫杆菌属,与嗜酸硫杆菌属KUT型菌株的相似性最大,为96.6%。菌株S30A2T的基因组DNAG+C含量为59.25mol%。菌株S30A2T和A.caldusKUT之间的平均核苷酸同一性ANIb和ANIm值分别为70.95和89.78%,数字DNA-DNA杂交值分别为24.9%。菌株S30A2T是严格需氧的,可以利用元素硫和四硫酸盐来支持化学营养生长。S30A2T的主要细胞脂肪酸为C19:1ω7c。呼吸醌是泛醌-8和泛醌-7。基于它的系统发育,遗传,表型,生理和化学分类学特征,菌株S30A2T被认为代表了酸性硫杆菌属的一种新物种,其名称为嗜酸硫杆菌。11月。是提议的。菌株类型为S30A2T(=CGMCC1.17059T=KCTC72580T)。
Strain S30A2T, isolated from the acid mine drainage sediment of Mengzi Copper Mine, Yunnan, is proposed to represent a novel species of the sulphur-oxidizing genus
Acidithiobacillus. Cells were Gram-stain-negative, non-endospore forming, highly motile with one or two monopolar flagella and rod-shaped. The strain was mesophilic, growing at 30-50 °C (optimum, 38 °C), acidophilic, growing at pH 2.0-4.5 (optimum, pH 2.5), and tolerant of 0-4 % (w/v; 684 mol l-1) NaCl. The 16S rRNA gene-based sequence analysis showed that strain S30A2T belongs to the genus
Acidithiobacillus and shows the largest similarity of 96.6 % to the type strain
Acidithiobacillus caldus KUT. The genomic DNA G+C content of strain S30A2T was 59.25 mol%. The average nucleotide identity ANIb and ANIm values between strain S30A2T and A. caldus KUT were 70.95 and 89.78 %, respectively and the digital DNA-DNA hybridization value was 24.9 %. Strain S30A2T was strictly aerobic and could utilize elementary sulphur and tetrathionate to support chemolithotrophic growth. The major cellular fatty acid of S30A2T was C19 : 1ω7c. The respiratory quinones were ubiquinone-8 and ubiquinone-7. Based upon its phylogenetic, genetic, phenotypic, physiologic and chemotaxonomic characteristics, strain S30A2T is considered to represent a novel species of the genus
Acidithiobacillus, for which the name
Acidithiobacillus acidisediminis sp. nov. is proposed. The type strain is S30A2T (=CGMCC 1.17059T=KCTC 72580T).