■喹硫平(QET)滥用由于其抗焦虑和享乐作用而增加,需要保护性辅助治疗。相思(A.saligna)花,用于传统医学,有潜在的健康益处。
■为了研究A.saligna花提取物对QET诱导的性毒性的保护作用,并通过代谢组学和生理学研究阐明可能的潜在机制。
■A.通过高分辨率超高效液相色谱-质谱(UPLC-ESI-qTOF-MS)对saligna提取物进行代谢物分析。将48只成年雄性白化病大鼠分为6组,共30天。海绵体内压(ICP),精液,生物化学,荷尔蒙,组织学,进行遗传和蛋白质印迹(WB)分析。
■A.saligna提取物富含酚类化合物,黄酮类化合物,单宁,和不饱和脂肪酸。QET显着降低ICP并对精液参数产生负面影响。A.saligna减轻了QET-55组中精子活力的降低并改善了过表达的促炎基因。A.saligna改善了抗氧化剂生物标志物的减少,睾丸激素,黄体生成素(LH),和卵泡刺激素(FSH),同时下调核因子κB(NF-κB)蛋白。A.saligna抵消了组织学检查显示的睾丸和前列腺结构的破坏。
■来自A.saligna的提取物,其中含有高浓度的抗氧化剂和抗炎化学物质,有效缓解QET引起的性毒性。这项研究提供了第一个已知的解释,即通过生物学,生物化学,和组织学参数。结果强调了A.saligna作为预防药物引起的性毒性的保护剂的潜力。
UNASSIGNED: Quetiapine (QET) abuse has increased due to its anxiolytic and hedonic effects, necessitating protective adjunct treatments. Acacia saligna (A. saligna) flowers, used in traditional medicine, have potential health benefits.
UNASSIGNED: To investigate the protective role of A. saligna flower extract against QET-induced sexual toxicity, and to elucidate the possible underlying mechanisms through metabolomic and physiological studies.
UNASSIGNED: A. saligna extract was subjected to metabolite profiling via High-Resolution Ultra-Performance Liquid Chromatography-Mass Spectrometry (UPLC-ESI-qTOF-MS). Forty-eight adult male albino rats were assigned into six groups for 30 days. The intracavernosal pressure (ICP), semen, biochemical, hormonal, histological, genetic and Western blot (WB) analyses were determined.
UNASSIGNED: A. saligna extract is rich in phenolic compounds, flavonoids, tannins, and unsaturated fatty acids. QET significantly decreased ICP and negatively affected semen parameters. A. saligna mitigated decreased sperm motility and ameliorated overexpressed proinflammatory genes in QET-55 group. A. saligna ameliorated the reduction of the antioxidant biomarkers, testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH), concurrent with downregulation of the nuclear factor kappa B (NF-κB) protein. A. saligna counteracted the disrupted testicular and prostatic structures revealed by histological examination.
UNASSIGNED: The extract from A. saligna, which contains a high concentration of antioxidants and anti-inflammatory chemicals, effectively mitigates sexual toxicity caused by QET. This study provided the first known explanation of the hypothesized processes behind the protective properties of A. saligna through biological, biochemical, and histological parameters. The results emphasize the potential of A. saligna as a safeguarding agent against drug-induced sexual toxicity.