四种未描述的聚酮化合物,beshanzoneA(1)和B(2)以及beshanhexanolsA(3)和B(4),从与极度濒危的中国特有针叶树冷杉相关的两种内生真菌的水稻发酵中分离出三种已知的(5-7)。γ-丁内酯衍生物1、2和5分离自Phomopsissp。BSZ-AZ-2,一种有趣的菌株,这次引起了我们的注意。在对青霉菌BSZ-P-4-1的后续研究中获得了环己醇衍生物3、4、6和7。化合物1-4的化学结构包括绝对构型通过光谱法测定,Mo2(OAc)4诱导电子圆二色性(IECD),GIAONMR计算和DP4+概率分析。特别是,化合物2包含一个新的5/5双环系统,其可以通过水解随后进行Aldol反应从已知化合物5生物源衍生。评估所有分离株对一小组细菌和真菌病原体的抗微生物活性。化合物6和7对白色念珠菌表现出中等抑制活性,MIC值为16和32μg/mL,分别。
Four undescribed polyketides, beshanzones A (1) and B (2) as well as beshanhexanols A (3) and B (4), along with three known ones (5-7) were isolated from the rice fermentation of two endophytic fungi associated with the critically endangered Chinese endemic conifer Abies beshanzuensis. γ-Butyrolactone derivatives 1, 2, and 5 were isolated from Phomopsis sp. BSZ-AZ-2, an interesting strain that drawn our attention this time. The cyclohexanol derivatives 3, 4, 6, and 7 were obtained during a follow-up investigation on Penicillium commune BSZ-P-4-1. The chemical structures including absolute configurations of compounds 1-4 were determined by spectroscopic methods, Mo2(OAc)4 induced electronic circular dichroism (IECD), GIAO NMR calculations and DP4+ probability analyses. In particular, compound 2 contains a novel 5/5 bicyclic ring system, which might be biogenetically derived from the known compound 5 through hydrolysis followed by an Aldol reaction. All isolates were evaluated for their antimicrobial activities against a small panel of bacterial and fungal pathogens. Compounds 6 and 7 showed moderate inhibitory activities against Candida albicans, with MIC values of 16 and 32 μg/mL, respectively.