在种质资源收集的鹰嘴豆种质和哈萨克斯坦的品种中研究了耐盐性。NaCl处理后,基因型之间存在显着差异,可以分为三组。表现最差的是第1组,包括叶绿素含量最低的五个ICC种质,最高的叶片坏死(LN),Na+积累,丙二醛(MDA)含量,和低谷胱甘肽比率GSH/GSSG。两个品种,Privo-1和Tassay,代表第2组,在这些特征上是中等的,而最好的表现是第3组,包含另外两个品种,Krasnokutsky-123和Looch,被发现主要是绿色植物和完全相反的特征模式。6KDArT标记与四个性状之间的标记性状关联(MTA)(LN,Na+,MDA,和GSH/GSSG)揭示了鹰嘴豆基因组中可能存在四个可能的候选基因,这些基因可能与三组相关。一个基因,ATP结合盒,CaABCC6被选中,和三种单倍型,A,在来自三个组的植物中鉴定D1和D2。发现第3组的两个最耐盐的品种具有单倍型D2,并具有新的鉴定的SNP。RT-qPCR分析证实,在单倍型D2的亲本和育种系植物中,NaCl处理后该基因强烈表达。种子蛋白的质谱显示谷胱甘肽还原酶和S-转移酶的积累较高,但不是过氧化物酶,在D2单倍型中。总之,假设CaABCC6基因与通过谷胱甘肽代谢对氧化应激的更好反应有关,而其他候选基因可能参与叶绿素含量和Na积累的控制。
Salinity tolerance was studied in chickpea accessions from a germplasm collection and in cultivars from Kazakhstan. After NaCl treatment, significant differences were found between genotypes, which could be arranged into three groups. Those that performed poorest were found in group 1, comprising five ICC accessions with the lowest chlorophyll content, the highest leaf necrosis (LN), Na+ accumulation, malondialdehyde (MDA) content, and a low glutathione ratio GSH/GSSG. Two cultivars, Privo-1 and Tassay, representing group 2, were moderate in these traits, while the best performance was for group 3, containing two other cultivars, Krasnokutsky-123 and Looch, which were found to have mostly green plants and an exact opposite pattern of traits. Marker-trait association (MTA) between 6K DArT markers and four traits (LN, Na+, MDA, and GSH/GSSG) revealed the presence of four possible candidate genes in the chickpea genome that may be associated with the three groups. One gene, ATP-binding cassette, CaABCC6, was selected, and three haplotypes, A, D1, and D2, were identified in plants from the three groups. Two of the most salt-tolerant cultivars from group 3 were found to have haplotype D2 with a novel identified SNP. RT-qPCR analysis confirmed that this gene was strongly expressed after NaCl treatment in the parental- and breeding-line plants of haplotype D2. Mass spectrometry of seed proteins showed a higher accumulation of glutathione reductase and S-transferase, but not peroxidase, in the D2 haplotype. In conclusion, the CaABCC6 gene was hypothesized to be associated with a better response to oxidative stress via glutathione metabolism, while other candidate genes are likely involved in the control of chlorophyll content and Na+ accumulation.