背景:帕金森病(PD),最普遍的帕金森病类型,是一种进行性神经系统疾病,其特征是一系列运动和非运动症状。PD的复杂病因被认为涉及衰老的总结,遗传易感性,和环境变量。然而,α-突触核蛋白在疾病的病理生理中起着重要作用。
方法:将UAS-α-Syn和Ddc-Gal4菌株杂交以产生称为PD蝇的后代。苍蝇的整个种群分为五组,每个有大约100只苍蝇和五个重复。对照组(w1118)和不接受治疗的PD组暴露于无月桂酸(LA)/左旋多巴(LD)饮食,而接受治疗的PD组饲喂250mg/kg的LA饮食,250毫克/千克LD饮食,或两者的组合持续21天。长寿,地轴,除其他生化测试外,还进行了嗅觉测定。
结果:由于α-突触核蛋白的过表达,机车容量,寿命,抗氧化状态均显著降低(p<0.05),细胞凋亡和神经炎症活动增加。然而,大多数处理的苍蝇显著改善(p<.05)。
结论:洛杉矶,无论是否与LD结合,在α-突触核蛋白/多巴脱羧酶基因修饰的果蝇帕金森病模型中引起了显着反应。
BACKGROUND: Parkinson\'s disease (PD), the most prevalent type of Parkinsonism, is a progressive neurological condition characterized by a range of motor and non-motor symptoms. The complicated etiology of PD is thought to involve a summation of aging, genetic predisposition, and environmental variables. However, the α-synuclein protein plays a significant role in the disease\'s pathophysiology.
METHODS: The UAS-α-Syn and Ddc-Gal4 strains were crossed to produce offspring referred to as PD flies. The entire population of flies was divided into five groups, each having about 100 flies and five replicates. The control group (w1118) and the PD group not receiving treatment were exposed to lauric acid (LA)/levodopa (LD)-free diet, while the PD groups that received treatments were fed with either a 250 mg/kg LA diet, a 250 mg/kg LD diet, or a combination of the two for 21 days. Longevity, geotaxis, and olfactory assays were performed in addition to other biochemical tests.
RESULTS: As a result of the overexpression of α-synuclein, the locomotive capacity, lifespan, and antioxidant status were all significantly (p < .05) reduced, and the apoptotic and neuroinflammatory activities were increased. Nevertheless, the majority of the treated flies improved significantly (p < .05).
CONCLUSIONS: LA, whether combined with LD or not, elicited a significant response in α-synuclein/dopa decarboxylase genetically modified Drosophila melanogaster Parkinsonism models.