背景:目前,对于慢性胰腺炎(CP),没有任何特定的治疗方法。目的探讨褪黑素给药对内质网应激的影响,氧化应激,纤维化,生化和组织病理学参数,和实验大鼠CP模型中的Abcc2、Abcc5和Abcg2基因水平。
方法:40只大鼠随机分为5组:假,CP,CP+25mg/kg褪黑素,CP+50mg/kg褪黑素,和CP+安慰剂。在所有的老鼠中,除了假组外,通过腹膜内注射caerulein建立了慢性胰腺炎模型。在治疗组中,褪黑激素被用作治疗剂。血清TGF-β,TNF-α,研究了MDA和GPx水平。对胰腺组织进行组织病理学评价。αSma的表达水平,IR1α,Perk,用qRT-PCR测量Abcc2、Abcc5和Abcg2基因。
结果:与CP和安慰剂组相比,褪黑激素组的生化结果显示出有利的变化。αSma,IR1α,褪黑素组的Perk表达水平显著较低。CP组Abcc2、Abcc5、Abcg2的表达程度显著高于假手术组,与CP组相比,褪黑素组的这些基因水平显着降低(分别为p<0.01,p<0.05,p<0.05)。
结论:鉴于这些有利的积极结果,褪黑素可能是CP过程中有用的预防剂。
BACKGROUND: Currently, there is not any specific treatment for chronic pancreatitis (CP). It was aimed to investigate the effects of melatonin administration on endoplasmic reticulum (ER) stress, oxidative stress, fibrosis, biochemical and histopathological parameters, and Abcc2,Abcc5, and Abcg2 gene levels in an experimental rat CP model.
METHODS: Forty rats were randomized into five groups: Sham, CP, CP+25 mg/kg melatonin, CP+50 mg/kg melatonin, and CP+placebo. In all rats, except the sham group, a model of chronic pancreatitis was accomplished with intraperitoneal caerulein administration. In treatment groups, melatonin was used as a therapeutic agent. Serum TGF-β, TNF-α, MDA and GPx levels were studied. Pancreatic tissues were evaluated histopathologically. The expression levels of αSma,IR1α,Perk,Abcc2,Abcc5, and Abcg2 genes were measured with the qRT-PCR.
RESULTS: Biochemical results of the melatonin groups exhibited favorable changes compared to the CP and placebo groups. αSma,IR1α,Perk expression levels were significantly lower in the melatonin groups. The expression levels of Abcc2, Abcc5, and Abcg2 were significantly higher in the CP group compared to the sham group, and these gene levels were significantly lower in the melatonin groups compared to the CP group (p < 0.01, p < 0.05, p < 0.05, respectively).
CONCLUSIONS: In light of these favorable positive results, melatonin may be a useful preventive agent in the course of CP.