背景:氧化/抗氧化剂失衡被认为是卵巢储备功能降低(DOR)的原因。据报道,8-羟鸟嘌呤DNA糖基化酶(OGG1)通过非催化结合启动子区域的氧化诱导的DNA损伤而充当抗氧化剂。
目的:本研究旨在评估有或没有DOR患者的血清OGG1浓度,并探讨OGG1作为DOR新诊断指标的临床价值。
方法:纳入武汉大学人民医院生殖医学中心64例DOR患者和78例卵巢储备功能正常(NOR)患者。使用酶联免疫吸附测定(ELISA)试剂盒测定患者月经周期2-5天的血清OGG1水平。有关入选患者的数据也从医院的数据库中获得,包括年龄,体重指数(BMI),抗苗勒管激素(AMH),等。结果:DOR组OGG1水平升高(2.08±0.70vs1.46±0.47nmol/L,P<0.001),与AMH水平呈负相关(Spearmanr=-0.586,P<0.001)。在调整了年龄和BMI后,OGG1与AMH呈负相关(β=-0.619,P<0.001)。ROC曲线分析表明,1.765nmol/L的截断值对区分有和没有DOR的个体具有适当的敏感性(81.30%)和特异性(76.90%)。曲线下面积(95%CI)为0.870(0.814至0.926),P<0.001。
结论:我们确定血清OGG1水平可作为DOR的新诊断指标。
Oxidative/antioxidant imbalance is considered a causal cause of diminished ovarian reserve (DOR). 8-oxyguanine DNA glycosylase (OGG1) has been reported to act as an antioxidant by binding non-catalytically to oxidation-induced DNA damage in the promoter region.
This study aimed to evaluate serum OGG1 concentrations in patients with or without DOR and to explore the clinical value of OGG1 as a novel diagnostic indicator for DOR.
Sixty-four women with DOR and seventy-eight women with normal ovarian reserve (NOR) from the reproductive medical center of Renmin Hospital of Wuhan University were included. Enzyme-linked immunosorbent assay (ELISA) kits were used to determine serum OGG1 levels in patients on 2-5 days of the menstrual cycle. Data regarding the enrolled patients were also obtained from the database of the hospital, including age, body mass index (BMI), anti-Müllerian hormone (AMH), etc. Results: OGG1 levels were increased in the DOR group (2.08 ± 0.70 vs 1.46 ± 0.47 nmol/L, P < 0.001) and negatively correlated with AMH levels (Spearman r = -0.586, P < 0.001). After adjusting for age and BMI, a negative association between OGG1 and AMH remained (β = -0.619, P < 0.001). ROC curve analysis showed that a cut-off value of 1.765 nmol/L had an appropriate sensitivity (81.30%) and specificity (76.90%) for discriminating individuals with and without DOR, with the area under the curve (95% CI) of 0.870 (0.814 to 0.926), P < 0.001.
We determined that serum OGG1 levels might be suggested as a new diagnostic indicator for DOR.