背景:紫外线-A辐射(UVA)通过产生炎症和氧化损伤来促进光老化/光致癌作用。当前的光保护策略受到UVA滤波器的可用性/利用率的限制。强调未满足的需求。大麻二酚(CBD),通过调节NFR-2、HMOX1和PPAR-y具有抗炎/抗氧化特性,可能会减轻UVA暴露造成的损害。
目标:前瞻性,单中心,试点临床试验(NCT05279495)。19名参与者随机应用纳米CBD(nCBD)或载体(VC)乳膏,失明的臀部部位每天两次,持续14天,然后用≤3xUVA最小红斑剂量照射治疗部位。24小时后,进行组织学穿刺活检,免疫组织化学,实时PCR。
结果:在24小时,21%的参与者在CBD治疗的皮肤上观察到的红斑少于VC皮肤。组织学上,nCBD治疗的皮肤比VC减少了UVA诱导的表皮增生(p=0.01)。与VC相比,免疫组织化学检测到nCBD处理的皮肤中细胞质/核8-氧代鸟嘌呤糖基化酶1染色减少(p<0.01)。定量mtDNAPCR表明,与VC相比,通过体内nCBD治疗,UVA诱导的ND4缺失(代理:4977bp缺失;p=0.003)和ND1(代理:3895bp缺失;p=0.002)显着减少。
结论:样本量。
结论:局部应用nCBD乳膏减少了UVA诱导的常见诱变性核DNA碱基损伤的形成,并防止了与UVA诱导的皮肤老化相关的mtDNA突变。这项试验是第一个确定含有CBD的局部药物在人类中的紫外线防护能力的试验。
BACKGROUND: UV-A radiation contributes to photoaging/photocarcinogenesis by generating inflammation and oxidative damage. Current photoprotective strategies are limited by the availability/utilization of UV-A filters, highlighting an unmet need. Cannabidiol (CBD), having anti-inflammatory/antioxidant properties via regulation of nuclear erythroid 2-related factor, heme oxygenase 1, and peroxisome proliferator-activated receptor gamma, could potentially mitigate damage from UV-A exposure.
OBJECTIVE: This is a prospective, single-center, pilot clinical trial (NCT05279495). Nineteen participants applied nano-CBD (nCBD) or vehicle (VC) cream to randomized, blinded buttock sites twice daily for 14 days; then, the treated sites were irradiated with ≤3× UV-A minimal erythema dose. After 24 hours, punch biopsies were obtained for histology, immunohistochemistry, and real-time polymerase chain reaction.
RESULTS: At 24 hours, 21% of participants had less observed erythema on CBD-treated skin than on VC skin. Histologically, nCBD-treated skin had reduced UV-A-induced epidermal hyperplasia than VC (P = .01). Immunohistochemistry detected reduced cytoplasmic/nuclear 8-oxoguanine glycosylase 1 staining in nCBD-treated skin compared with VC (P < .01). Quantitative mtDNA polymerase chain reaction demonstrated that UV-A-induced deletion of ND4 (proxy:4977 bp deletion; P = .003) and ND1 (proxy:3895 bp deletion; P = .002) was significantly reduced by in vivo nCBD treatment compared with VC.
CONCLUSIONS: Small sample size is this study\'s limitation.
CONCLUSIONS: Topically applied nCBD cream reduced UV-A-induced formation of a frequent mutagenic nuclear DNA base lesion and protected against mtDNA mutations associated with UV-A-induced skin aging. To our knowledge, this trial is the first to identify UV-protective capacity of CBD-containing topicals in humans.