5-羟色胺(5-HT)和/或去甲肾上腺素参与中枢神经系统(CNS)的急性瘙痒过程已报道使用抗抑郁药,比如米纳西普兰,血清素和去甲肾上腺素再摄取抑制剂,还有米氮平,去甲肾上腺素能和特定的5-羟色胺能抗抑郁药;然而,中枢神经系统尚未完全阐明5-HT受体家族在急性瘙痒过程中的作用。在本研究中,米那普仑或米氮平改善了氯喹(CQ)引起的抓挠行为,这些作用被5-HT4拮抗剂SB207266逆转,或者SB258585,一种5-HT6拮抗剂,但不是由SB258585,一种5-HT5拮抗剂。此外,鞘内注射5-HT4激动剂可减轻CQ诱导的划痕,如BIMU8和ML10302,以及5-HT6激动剂,WAY208466。相反,组胺诱导的划痕不受5-HT4激动剂或5-HT6激动剂的影响。同样,5-HT4,5-HT5或5-HT6受体拮抗剂不能逆转这些抗抑郁药对组胺诱导的抓痕的改善.因此,5-HT参与米纳普仑和米氮平改善CQ诱导的划痕,和5-HT4,5-HT5和5-HT6受体在施用CQ或组胺后的急性瘙痒过程中起着不同的作用。
The involvement of serotonin (5-HT) and/or noradrenaline in acute pruriceptive processing in the central nervous system (CNS) has been reported using antidepressants, such as milnacipran, a serotonin and noradrenaline reuptake inhibitor, and mirtazapine, a noradrenergic and specific serotonergic antidepressant; however, the roles of 5-HT receptor family in acute pruriceptive processing have not been fully elucidated in the CNS. In the present study, scratching behavior induced by chloroquine (CQ) was ameliorated by milnacipran or mirtazapine, and these effects were reversed by SB207266, a 5-HT4 antagonist, or SB258585, a 5-HT6 antagonist, but not by SB258585, a 5-HT5 antagonist. Moreover, CQ-induced scratches were mitigated by intrathecal injection of 5-HT4 agonists, such as BIMU8 and ML10302, and the 5-HT6 agonist, WAY208466. Conversely, histamine-induced scratches were not affected by the 5-HT4 agonists or a 5-HT6 agonist. Similarly, the amelioration of histamine-induced scratches by these antidepressants was not reversed by the 5-HT4, 5-HT5, or 5-HT6 receptor antagonist. Therefore, 5-HT is involved in the amelioration of CQ-induced scratches by milnacipran and mirtazapine, and 5-HT4, 5-HT5, and 5-HT6 receptors play differential roles in acute pruriceptive processing after administration of CQ or histamine.