该研究旨在描述家猫子宫管的形态发生,从肾旁导管(TsPD)的原始输卵管段到成熟器官。具体目标包括确定漏斗壁层发育速度和方式的差异,壶腹,还有地峡.在这项研究中,我们对子宫管壁进行了光学显微镜(LM)观察,和三维模型的分析,首次用于可视化旁肾小管的排列并突出显示正在发育的粘膜褶皱的模式。结果揭示了家猫子宫管产前发育的两个阶段。第一阶段从第26-44p.c.(受孕后)持续,并包括TsPD的生长。它开始于在中肾前部的间质中形成TsPD的上皮小管。小管,被间充质包围,向后伸长,TsPD可以根据其位置和直径分为三个部分,即,前,中间,和后路。第一阶段以间充质的组织分化为松散的结缔组织和粘膜形成结束。第二阶段从大约第45-63天开始,并包括在子宫管壁中形成适当的层。层发育的速度和模式特定于子宫管的每个部分,因为组织首先在其前部分化,然后向后继续。粘膜褶皱在第44天后出现。在漏斗中,在产前期结束时,它们变得又长又分支。然后,大约下午48天,单个粘膜褶皱出现在壶腹,最后,即,大约下午63天,它们在地峡变得可见。3D模型揭示了壶腹粘膜褶皱的独特螺旋形排列。肌层开始发育大约第48-51天,在子宫管的前部。大约在下午55天,可以在壶腹中区分圆形肌肉层,而在地峡,它在出生前发育。漏斗的出生后发育包括粘膜褶皱的迷宫样系统的形成,而在壶腹和地峡,分支的粘膜褶皱和第二个纵向肌肉层在出生后发育。在产前期结束时,子宫管各个部分之间的壁厚差异反映了成熟女性子宫管壁的适当比例。
The study aimed to describe the morphogenesis of the domestic cat uterine tube, from the primordial tubal segment of the paramesonephric duct (TsPD) to the mature organ. The specific aims included identifying differences in the rate and way of development of the layers in the wall of the infundibulum, the ampulla, and the isthmus. In the study we conducted light microscopic (LM) observations of the uterine tube wall, and analysis of 3D models, which were used for the first time to visualize the arrangement of the paramesonephric duct and highlight the pattern of the developing mucosal folds. The results revealed the two stages in the prenatal development of the domestic cat uterine tube. The first stage lasts from day 26-44 p.c. (post-conception) and includes the growth of the TsPD. It starts with formation of the TsPD\'s epithelial tubule in the mesenchyme of the anterior part of the mesonephros. The tubule, surrounded by mesenchyme, elongates posteriorly, and the TsPD can be divided into three sections according to their location and diameter, i.e., anterior, middle, and posterior. The first stage ends with the histodifferentiation of the mesenchyme into loose connective tissue and mucosa formation. The second stage lasts from approximately day 45-63 p.c. and comprises the formation of the proper layers in the uterine tube wall. The pace and pattern of layers\' development are specific to each section of the uterine tube, as tissues first differentiate in its anterior part and then continue posteriorly. The mucosal folds appear after day 44 p.c. in the infundibulum, and they become long and branched by the end of the prenatal period. Then, approximately day 48 p.c., the single mucosal folds appear in the ampulla, and at the end, i.e., about day 63 p.c., they become visible in the isthmus. The 3D models revealed the unique spiral-shaped arrangement of the mucosal folds in the ampulla. The tunica muscularis starts to develop ca day 48-51 p.c. in the anterior part of the uterine tube. On approximately day 55 p.c., the circular muscle layer can be distinguished in the ampulla, while in the isthmus, it develops just before birth. The postnatal development of the infundibulum includes the formation of the labyrinth-like system of mucosal folds, whereas in the ampulla and isthmus, branched mucosal folds and the second longitudinal muscle layer develop postnatally. At the end of the prenatal period, the differences in the wall thickness between the individual segments of the uterine tube reflect the proper proportions of the uterine tube wall in the mature female.