■在老化的皮肤中,活性氧(ROS)诱导细胞外基质(ECM)的降解,导致明显的衰老迹象。ECM中的胶原被基质金属蛋白酶(MMP)裂解。丁香草醇(SYR),从人参浆果中分离,有各种生理活动,包括抗炎作用。然而,SYR通过抗氧化和自噬调节的抗衰老作用尚未阐明。
在H2O2存在下在人HaCaT角质形成细胞中研究了SYR对皮肤老化的预防作用,并用SYR(0-200μg/mL)处理角质形成细胞。通过实时PCR和蛋白质印迹法测定MMP-2和-9的mRNA和蛋白水平,分别。通过2,2二苯基-1-吡啶酰肼(DPPH)和2,2'-氮杂-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)测定法研究了自由基清除活性。通过Western印迹和共聚焦显微镜评估LC3B水平。
■SYR显著降低H2O2处理和未处理的HaCaT细胞中MMP-9和-2的基因表达和蛋白质水平。SYR未显示对HaCaT细胞的细胞毒性。SYR表现出DPPH和ABTS自由基清除活性,EC50值为10.77和10.35μg/mL,分别。SYR提高了H2O2刺激的HaCaT细胞中内源性和外源性LC3B的总水平。3-甲基腺嘌呤(3-MA),自噬抑制剂,SYR对MMP-2表达的抑制作用。
■SYR在H2O2刺激的HaCaT细胞中显示出抗氧化活性和上调的自噬活性,降低MMP-2和MMP-9的表达与皮肤老化有关。我们的结果表明,SYR具有作为预防皮肤老化的化妆品添加剂的潜在价值。
UNASSIGNED: In aged skin, reactive oxygen species (ROS) induces degradation of the extracellular matrix (ECM), leading to visible aging signs. Collagens in the ECM are cleaved by matrix metalloproteinases (MMPs). Syringaresinol (SYR), isolated from Panax ginseng berry, has various physiological activities, including anti-inflammatory action. However, the anti-aging effects of SYR via antioxidant and autophagy regulation have not been elucidated.
UNASSIGNED: The preventive effect of SYR on skin aging was investigated in human HaCaT keratinocytes in the presence of H2O2, and the keratinocyte cells were treated with SYR (0-200 μg/mL). mRNA and protein levels of MMP-2 and -9 were determined by real-time PCR and Western blotting, respectively. Radical scavenging activity was researched by 2,2 diphenyl-1-picrylhydrazyl (DPPH) and 2,2\'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays. LC3B level was assessed by Western blotting and confocal microscopy.
UNASSIGNED: SYR significantly reduced gene expression and protein levels of MMP-9 and -2 in both H2O2-treated and untreated HaCaT cells. SYR did not show cytotoxicity to HaCaT cells. SYR exhibited DPPH and ABTS radical scavenging activities with an EC50 value of 10.77 and 10.35 μg/mL, respectively. SYR elevated total levels of endogenous and exogenous LC3B in H2O2-stimulated HaCaT cells. 3-Methyladenine (3-MA), an autophagy inhibitor, counteracted the inhibitory effect of SYR on MMP-2 expression.
UNASSIGNED: SYR showed antioxidant activity and up-regulated autophagy activity in H2O2-stimulated HaCaT cells, lowering the expression of MMP-2 and MMP-9 associated with skin aging. Our results suggest that SYR has potential value as a cosmetic additive for prevention of skin aging.