为了研究桂皮醛对利血平诱导的帕金森病(PD)大鼠模型的神经保护机制,将72只雄性Wistar大鼠随机分为空白组,模型组,Madopar集团,肉桂醛高,medium-,和低剂量组。除了空白组,其余各组每隔一上午腹腔注射1次利血平0.1mg·kg~(-1),每天下午进行肉桂醛和Madopar溶液的充气。开放式现场测试,旋转杆试验,并在实验中进行口腔咀嚼运动评价。大脑被取出并修复。TSA检测多巴胺受体D1(DRD1)的阳性表达,通过酶联免疫吸附试验(ELISA)检测脑内多巴胺(DA)和3,4-二羟基苯乙酸(DOPAC)等神经递质的变化。RT-PCR和Westernblot检测黑质(SN)中酪氨酸羟化酶(TH)和α-突触核蛋白(α-Syn)的蛋白和mRNA表达水平。结果表明,注射利血平后,模型组的头发颜色变黄变脏;逮捕行为减弱,体重减轻了。自发运动和探索行为减少,协调运动能力下降。口腔咀嚼次数增加,但是认知能力下降了,SN中DRD1阳性表达区的比例降低。TH蛋白和mRNA表达下调,α-Syn蛋白和mRNA表达上调。肉桂醛干预后,对PD模型动物有明显疗效。自发的运动行为,停留在杆中的时间,运动的时间,移动的距离,站立次数增加,口腔咀嚼次数减少。SN中DRD1阳性表达区的比例增加,α-Syn蛋白和mRNA表达水平下调。TH蛋白和mRNA表达水平上调。此外,DA的水平,DOPAC,大脑中的高香草酸(HVA)神经递质上调。本研究可为临床治疗和预防PD提供新的实验依据。
In order to study the neuroprotective mechanism of cinnamaldehyde on reserpine-induced Parkinson\'s disease(PD) rat models, 72 male Wistar rats were randomly divided into blank group, model group, Madopar group, and cinnamaldehyde high-, medium-, and low-dose groups. Except for the blank group, the other groups were intraperitoneally injected with reserpine of 0.1 mg·kg~(-1) once every other morning, and cinnamaldehyde and Madopar solutions were gavaged every afternoon. Open field test, rotarod test, and oral chewing movement evaluation were carried out in the experiment. The brain was taken and fixed. The positive expression of dopamine receptor D1(DRD1) was detected by TSA, and the changes in neurotransmitters such as dopamine(DA) and 3,4-dihydroxyphenylacetic acid(DOPAC) in the brain were detected by enzyme-linked immunosorbent assay(ELISA). The protein and mRNA expression levels of tyrosine hydroxylase(TH) and α-synuclein(α-Syn) in substantia nigra(SN) were detected by RT-PCR and Western blot. The results showed that after the injection of reserpine, the hair color of the model group became yellow and dirty; the arrest behavior was weakened, and the body weight was reduced. The spontaneous movement and exploration behavior were reduced, and the coordination exercise ability was decreased. The number of oral chewing was increased, but the cognitive ability was decreased, and the proportion of DRD1 positive expression area in SN was decreased. The expression of TH protein and mRNA was down-regulated, and that of α-Syn protein and mRNA was up-regulated. After cinnamaldehyde intervention, it had an obvious curative effect on PD model animals. The spontaneous movement behavior, the time of staying in the rod, the time of movement, the distance of movement, and the number of standing times increased, and the number of oral chewing decreased. The proportion of DRD1 positive expression area in SN increased, and the protein and mRNA expression levels of α-Syn were down-regulated. The protein and mRNA expression levels of TH were up-regulated. In addition, the levels of DA, DOPAC, and homovanillic acid(HVA) neurotransmitters in the brain were up-regulated. This study can provide a new experimental basis for clinical treatment and prevention of PD.