17α-甲基睾酮(17MT),一种在污水中常见的合成有机化合物,会影响水生动物的繁殖,如罗非鱼和黄鲶鱼。在本研究中,雄性Gobiocyprisrarus暴露于25、50和100ng/L的17α-甲基睾丸激素(17MT)中7天。我们首先分析miRNA-和RNA-seq结果以确定miRNA-靶基因对,然后在17MT施用后开发miRNA-mRNA相互作用网络。总重量,总长度,体长和体长在实验组和对照组之间没有显着差异。将石蜡切片法应用于MT暴露组和对照组的G。我们发现对照组睾丸中成熟精子(S)较多,次级精母细胞(SSs)和精原细胞(SGs)较少。随着17MT浓度的增加,在雄性G.rarus的睾丸中观察到的成熟精子(S)越来越少。结果表明,FSH,11-KT,与对照组相比,暴露于25ng/L17MT的个体中E2和E2明显更高。VTG,FSH,LH,11-KT,与对照组相比,50ng/L17MT暴露组的E2和E2显着降低。VTG,FSH,LH,11-KT,在暴露于100ng/L17MT的组中,E2和T显着降低。高通量测序显示73,449个基因,1205种已知成熟miRNA,和939个新的miRNA在G.rarus的性腺中。使用miRNA-seq,49(MT25-Mvs.Con-M),66(MT50-Mvs.Con-M),和49(MT100-Mvs.在治疗组中鉴定了Con-M)DEM。五个成熟miRNA(miR-122-x,miR-574-x,miR-430-y,lin-4-x,和miR-7-y),以及七个差异表达基因(soat2,inhbb,ihhb,gatm,faxdc2,ebp,和cyp1a1),这可能与睾丸发育有关,新陈代谢,凋亡,和疾病反应,使用qRT-PCR进行分析。此外,miR-122-x(与脂质代谢有关),miR-430-y(胚胎发育)lin-4-x(凋亡),和miR-7-y(疾病)在17MT暴露的G.rarus的睾丸中差异表达。这项研究强调了miRNA-mRNA对在睾丸发育和对疾病的免疫反应的调节中的作用,并将促进对硬骨鱼繁殖的miRNA-RNA相关调节的未来研究。
17α-Methyltestosterone (17MT), a synthetic organic compound commonly found in sewage waters, can affect reproduction in aquatic animals, such as tilapia and yellow catfish. In the present study, male Gobiocypris rarus were exposed to 25, 50, and 100 ng/L of 17α-methyltestosterone (17MT) for 7 days. We first analyzed miRNA- and RNA-seq results to determine miRNA-target gene pairs and then developed miRNA-mRNA interactive networks after 17MT administration. Total weights, total lengths, and body lengths were not significantly different between the test groups and control groups. The paraffin slice method was applied to testes of G. rarus in the MT exposure and control groups. We found that there were more mature sperm (S) and fewer secondary spermatocytes (SSs) and spermatogonia (SGs) in the testes of control groups. As 17MT concentration increased, fewer and fewer mature sperm (S) were observed in the testes of male G. rarus. The results showed that FSH, 11-KT, and E2 were significantly higher in individuals exposed to 25 ng/L 17MT compared with the control groups. VTG, FSH, LH, 11-KT, and E2 were significantly lower in the 50 ng/L 17MT exposure groups compared to the control groups. VTG, FSH, LH, 11-KT, E2, and T were significantly lower in the groups exposed to 100 ng/L 17MT. High-throughput sequencing revealed 73,449 unigenes, 1205 known mature miRNAs, and 939 novel miRNAs in the gonads of G. rarus. With miRNA-seq, 49 (MT25-M vs. Con-M), 66 (MT50-M vs. Con-M), and 49 (MT100-M vs. Con-M) DEMs were identified in the treatment groups. Five mature miRNAs (miR-122-x, miR-574-x, miR-430-y, lin-4-x, and miR-7-y), as well as seven differentially expressed genes (soat2, inhbb, ihhb, gatm, faxdc2, ebp, and cyp1a1), which may be associated with testicular development, metabolism, apoptosis, and disease response, were assayed using qRT-PCR. Furthermore, miR-122-x (related to lipid metabolism), miR-430-y (embryonic development), lin-4-x (apoptosis), and miR-7-y (disease) were differentially expressed in the testes of 17MT-exposed G. rarus. This study highlights the role of miRNA-mRNA pairs in the regulation of testicular development and immune response to disease and will facilitate future studies on the miRNA-RNA-associated regulation of teleost reproduction.