■这项研究的目的是分析黄芪甲苷(AST)对糖尿病非愈合伤口(DNHW)的免疫微环境的调节作用,并分析创面多层修复的临床疗效和机制。
■90只成年雄性Wistar大鼠,在自然感染下保持健康(SPF),随机分为三组,即,空白,对照组和观察组,每组30只大鼠。适应性喂养7天后,建立糖尿病模型。模型形成后,伤口被均匀地准备好,然后只有空白组被剃光。对照组和观察组均采用湿润暴露疗法。对照组用生理盐水纱布覆盖,观察组采用AST纱布覆盖。1日观察并记录两组创面愈合情况,Seven,形成后第14天。并测定创面组织中α-平滑肌肌动蛋白(α-SMA)和I型胶原(COL-1)的含量。
■在伤口形成后的第1天,伤口愈合区,α-SMA,与COL-1水平在三组间具有一致性(p>0.05)。在伤口形成后的第7天和第14天,三组的伤口愈合面积在组内相比有所增加,但只有对照组和观察组的创面愈合面积明显高于创面形成后第1天(p<0.05)。此外,空白组α-SMA和COL-1水平较低,对照组和观察组α-SMA和COL-1水平较高(p<0.05)。在组间比较中,伤口愈合区,α-SMA,对照组和观察组的COL-1水平均高于空白组,而伤口愈合区域,α-SMA,观察组患者的COL-1水平高于对照组(p<0.05)。
■AST可以调节DNHW的免疫微环境,改善α-SMA和COL-1,加速DNHW的伤口愈合。
UNASSIGNED: The aim of the research was to analyse the regulatory effect of astragaloside (AST) on the immune microenvironment of diabetic non-healing wound (DNHW), and to analyse the clinical efficacy and mechanism of wound repair in multiple layers.
UNASSIGNED: Ninety adult male Wistar rats, which were kept healthy (SPF) under natural infection, were randomly divided into three groups, namely, blank, control and observation groups, with 30 rats in each group. After adaptive feeding for 7 days, the diabetes model was established. After the model was formed, the wounds were uniformly prepared, and then the blank group only was shaved. Both the control group and the observation group were treated with moist exposure therapy. The control group was covered with physiological saline gauze, while the observation group was covered with AST gauze. The healing status of the wounds in both groups was observed and recorded on the 1st, 7th, and 14th days after formation. And the levels of α-smooth muscle actin (α-SMA) and collagen I (COL-1) in the wound tissue were measured.
UNASSIGNED: On the 1st day after wound formation, the wound healing area, α-SMA, and COL-1 levels in the three groups were consistent (p > 0.05). On the 7th and 14th days after wound formation, the wound healing area in the three groups increased compared within the group, but only the control and observation groups had significantly higher wound healing area than on the 1st day after wound formation (p < 0.05). In addition, the blank group had lower levels of α-SMA and COL-1, while the control and observation groups had higher levels of α-SMA and COL-1 (p < 0.05). In the comparison between groups, the wound healing area, α-SMA, and COL-1 levels in the control and observation groups were higher than those in the blank group, while the wound healing area, α-SMA, and COL-1 levels in the observation group were higher than those in the control group (p < 0.05).
UNASSIGNED: AST can regulate the immune microenvironment of DNHW, improve α-SMA and COL-1, and accelerate the wound healing of DNHW.