所述毛癣菌复合体包含负责各种皮肤病感染的一组皮肤癣菌真菌。这种复杂的物种不断增加的抗药性,特别是对墨发毛癣菌的特比萘芬抗性,是皮肤科医生实践中的一个主要问题。这项研究提供了从顺化市-越南患者中分离出的T.mentagrosphytes复杂菌株的综合分析,关注它们的表型和遗传特征,抗真菌药敏谱和分子流行病学。从形态学和表型上鉴定了皮肤癣菌培养样品中的角质形成性真菌,通过ITS测序和系统发育分析确认的物种和基因型。进行抗真菌药敏试验以评估其对伊曲康唑的敏感性,伏立康唑和特比萘芬.24%(n=27/114)的浅表真菌病在表型上归因于T.T.interdigitale,主要是基因型II*,占主导地位(44.4%),其次是T.stagagrosphytes基因型III*(22.2%),吲哚科(14.8%),T、吨苏兰(11.2%),和T.植叶植物(7.4%)。虽然所有分离株对伊曲康唑和伏立康唑敏感,一半的吲哚虫分离株对特比萘芬表现出耐药性,与SQLE蛋白中的Phe397Leu突变有关。这项研究强调了越南存在耐特比萘芬T.indotineae分离株,强调有必要调查皮肤癣菌的耐药性,并在临床实践中采取有效措施。
从顺化市皮肤癣菌病中分离出的毛癣菌复合体中的物种多样性,越南,被观察到。越南首次检测到耐特比萘芬的吲哚毛癣菌,强调实施抗真菌药敏试验以有效管理和防止耐药分离株传播的重要性。
The Trichophyton mentagrophytes complex comprises a group of dermatophyte fungi responsible for various dermatological infections. The increasing drug resistance of this species complex, especially
terbinafine resistance of Trichophyton indotineae, is a major concern in dermatologist practice. This study provides a comprehensive analysis of T. mentagrophytes complex strains isolated from patients in Hue City, Vietnam, focusing on their phenotypic and genetic characteristics, antifungal susceptibility profiles, and molecular epidemiology. Keratinophilic fungi from dermatophytosis culture samples were identified morphologically and phenotypically, with species and genotypes confirmed by internal transcribed spacer sequencing and phylogenetic analysis. Antifungal susceptibility testing was carried out to evaluate their susceptibility to itraconazole, voriconazole, and
terbinafine. The 24% (n = 27/114) of superficial mycoses were phenotypically attributed to T. mentagrophytes complex isolates. Trichophyton interdigitale, mainly genotype II*, was predominant (44.4%), followed by T. mentagrophytes genotype III* (22.2%), T. indotineae (14.8%), T. tonsurans (11.2%), and T. mentagrophytes (7.4%). While all isolates were susceptible to itraconazole and voriconazole, half of T. indotineae isolates exhibited resistance to
terbinafine, linked to the Phe397Leu mutation in the SQLE protein. This study highlighted the presence of
terbinafine-resistant T. indotineae isolates in Vietnam, emphasizing the need to investigate dermatophyte drug resistance and implement effective measures in clinical practice.
Species diversity within the Trichophyton mentagrophytes complex isolated from dermatophytosis in Hue City, Vietnam, was observed.
Terbinafine-resistant T. indotineae isolates were detected for the first time in Vietnam, emphasizing the importance of implementing antifungal susceptibility testing to effectively manage and prevent the spread of resistant isolates.