赤霉素(GAs),在植物生长和发育中起重要作用的酶,它们在植物中的水平可以由赤霉素氧化酶(GAoxs)调节。作为重要的果树和观赏植物,对李属植物结构形成机制的研究至关重要。这里,从P.mume中鉴定出85个GAox基因,P.Armeniaca,P.水杨酸,还有P.Persica,他们被分为六个亚组。保守基序和基因结构分析表明,GAoxs在四个李属物种中保守。共线性分析揭示了P.mu基因组中PmGAoxs的两个片段复制事件。启动子顺式元件分析显示24个PmGAoxs包含激素响应元件和发育调控元件。表达谱表明PmGAoxs具有组织表达特异性,花蕾休眠期的GA水平由某些PmGAoxs控制。用IAA或GA3处理后,茎中PmGA2ox8的转录水平显着增加,并且在直立茎和哭泣茎之间显示出差异表达水平。在根中检测到由PmGA2ox8启动子驱动的GUS活性,茎,叶子,拟南芥的花器官。PmGA2ox8在拟南芥中的过表达导致矮化表型,莲座叶数量增加,但叶面积减少,延迟开花。我们的结果表明,GAoxs在李属物种中是保守的,PmGA2ox8在调节株高方面发挥了重要作用。
Gibberellins (GAs), enzymes that play a significant role in plant growth and development, and their levels in plants could be regulated by gibberellin-oxidases (GAoxs). As important fruit trees and ornamental plants, the study of the mechanism of plant architecture formation of the Prunus genus is crucial. Here, 85 GAox genes were identified from P. mume, P. armeniaca, P. salicina, and P. persica, and they were classified into six subgroups. Conserved motif and gene structure analysis showed that GAoxs were conserved in the four Prunus species. Collinearity analysis revealed two fragment replication events of PmGAoxs in the P. mume genome. Promoter cis-elements analysis revealed 24 PmGAoxs contained hormone-responsive elements and development regulatory elements. The expression profile indicated that PmGAoxs have tissue expression specificity, and GA levels during the dormancy stage of flower buds were controlled by certain PmGAoxs. After being treated with IAA or GA3, the transcription level of PmGA2ox8 in stems was significantly increased and showed a differential expression level between upright and weeping stems. GUS activity driven by PmGA2ox8 promoter was detected in roots, stems, leaves, and flower organs of Arabidopsis. PmGA2ox8 overexpression in Arabidopsis leads to dwarfing phenotype, increased number of rosette leaves but decreased leaf area, and delayed flowering. Our results showed that GAoxs were conserved in Prunus species, and PmGA2ox8 played an essential role in regulating plant height.