PDF(Pubmed)
Abstract：
Ethylene response factors have been shown to be involved in the effects of plant developmental processes and to regulate stress tolerance. The aim of this study was to recognize the regulatory mechanisms of ethylene response factors on tobacco plant height. In this study, a gene-edited mutant (ERF10-KO) and wild type (WT) were utilized as experimental materials. Transcriptome and metabolome analyses were used to investigate the regulatory mechanism of NtERF10 gene editing on plant height in tobacco. Here, through the analysis of differentially expressed genes (DEGs), 2051 genes were upregulated and 1965 genes were downregulated. We characterized the different ERF10-KO and WT plant heights and identified key genes for photosynthesis, the plant hormone signal transduction pathway and the terpene biosynthesis pathway. NtERF10 was found to affect the growth and development of tobacco by regulating the expression levels of the PSAA, PSBA, GLY17 and GGP3 genes. Amino acid metabolism was analyzed by combining analyses of differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs). In addition, we found that members of the bHLH, NAC, MYB, and WRKY transcription factor families have vital roles in regulating plant height. This study not only provides important insights into the positive regulation of the ethylene response factor NtERF10 on plant height during plant growth and development but also provides new research ideas for tobacco molecular breeding.
摘要：
乙烯反应因子已被证明参与植物发育过程的影响并调节胁迫耐受性。本研究的目的是认识乙烯响应因子对烟草株高的调控机制。在这项研究中,利用基因编辑的突变体(ERF10-KO)和野生型(WT)作为实验材料。通过转录组和代谢组分析研究了NtERF10基因编辑对烟草株高的调控机制。这里,通过对差异表达基因(DEGs)的分析，2051个基因上调，1965个基因下调。我们表征了不同的ERF10-KO和WT植物高度，并确定了光合作用的关键基因，植物激素信号转导途径和萜烯生物合成途径。发现NtERF10通过调节PSAA的表达水平影响烟草的生长发育,PSBA,GLY17和GGP3基因。通过结合差异表达基因（DEGs）和差异积累代谢物（DAMs）的分析来分析氨基酸代谢。此外,我们发现bHLH的成员，NAC,MYB,和WRKY转录因子家族在调节株高中起着至关重要的作用。本研究不仅为乙烯反应因子NtERF10在植物生长发育过程中对株高的正向调控提供了重要的见解，而且为烟草分子育种提供了新的研究思路。
